枯草芽孢杆菌发酵培养基的优化  

Optimization of Bacillus subtilis B908 Fermentation Medium

作　　者：张丽霞[1] 李荣禧[1] 王琦[2] 梅汝鸿[2] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010018 [2]中国农业大学植物病理学系,北京100094

出　　处：《中国生物防治》2006年第S1期82-88,共7页Chinese Journal of Biological Control

基　　金：国家863科技攻关项目

摘　　要：在摇瓶发酵条件下,采用Plackett-Burman设计法和响应面分析法对枯草芽孢杆菌B908菌株的培养基配方进行优化,确定了影响B908菌株发酵液含菌量的3个重要因子依次为玉米粉、碳酸钙和豆饼粉;对这3个因子进行爬坡路径试验,通过响应面分析法确定了主要影响因子的最佳条件。优化后的培养基配方使摇瓶发酵液每毫升的含菌量从0.6×109个/ml提高到1.18×1010个/ml。Plackett-Burman design and response surface analysis were used to optimize the fermentation medium of Bacillus subtilis B908.Based on the results of Plackett-Burman design,important factors affecting the yield of Bacillus subtilis B908 were corn flour,bean flour and CaCO_3.Then,the three factors were further optimized using response surface analysis.The optimized fermentation medium allowed the production to be increased from 6.6×10~9spores ml^(-1) to 1.18×10^(10)spores ml^(-1).

关 键 词：枯草芽孢杆菌 发酵培养基 PLACKETT-BURMAN 响应面分析法 

分 类 号：S476[农业科学—农业昆虫与害虫防治]