万隆霉素的高效液相色谱法测定  

Determination of Wanlongmycin by High Performance Liquid Chromatography

作　　者：张晓勇[1] 林壁润[1] 高向阳[2] 沈会芳[1] 

机构地区：[1]广东省农业科学院植物保护研究所,广州510640 [2]华南农业大学生命科学院,广州510642

出　　处：《中国生物防治》2006年第S1期78-81,共4页Chinese Journal of Biological Control

基　　金：国家自然科学基金(30471161);广东省自然科学基金(04002077)

摘　　要：采用反相高效液相色谱(HPLC)法测定发酵产物中万隆霉素的含量,色谱柱为C18反向分析柱(3.9mm×150mm),柱温为40℃,流动相为甲醇∶水(20∶80),检测波长为280nm,流速为1ml/min。结果表明万隆霉素在上述条件下能从发酵产物中获得很好地分离,万隆霉素浓度在17.5~280μg/ml范围内线性良好,线性回归方程为:y=17343x-164139,相关系数R2=0.9998(n=5)。The method for the separation and determination of Wanlongmycin in fermentation broth was developed.A C18 reversed column(3.9mm×150mm) was used.The column temperature was 40 ℃.The mobile phase was methanol-water(20∶80).The flow rate was 1.0ml/min and the detection wavelength was UV 280nm.The results showed that the active constituent Wanlongmycin can be separated effectively.In linear range 17.5-280μg/ml,there was a regressive equation y=17343x-164139,the correlation coefficient was 0.9998(n=5).

关 键 词：万隆霉素 高效液相色谱法 测定 

分 类 号：S476[农业科学—农业昆虫与害虫防治]