人胰岛素样生长因子-1基因治疗老龄大鼠阴茎勃起功能障碍的研究  

Molecular biology investigation of diabetes cystopathy in rats:relationship beween bladder relaxation characters and 3-adrenergic receptor level

作　　者：蒲小勇[1] 胡礼泉[1] 王行环[2] 韩雪[3] 叶林柏[3] 廖庆娇 王怀鹏[2] 李世文[1] 田斌群[1] 郑新民[1] 宋健 

机构地区：[1]武汉大学中南医院泌尿外科男科学研究中心,430071 [2]广东省人民医院泌尿外科 [3]武汉大学生命科学学院国家重点实验室 [4]武汉大学医学部解剖学系

出　　处：《中华泌尿外科杂志》2006年第S1期117-119,共3页Chinese Journal of Urology

摘　　要：目的探讨阴茎海绵体内注射胰岛素样生长因子1(hIGF-1)基因提高老年性大鼠阴茎勃起功能的可能性。方法9月龄>500gSD大鼠35只随机分为3组,注射PCDB-hIGF-1组15只,注射PCDB组10只,空白对照组10只。注射后1~2d测量大鼠海绵体内压(ICP),收集大鼠阴茎海绵体组织,Trizol方法提取总RNA,RT-PCR检测hIGF-1基因mRNA的表达,免疫组化检测hIGF-1蛋白表达。结果注射PCDB-hIGF-1组大鼠ICP(149±10)高于注射PCDB空质粒组(93±11)和对照组(89±12),差异有统计学意义(P<0.05);RT-PCR检测注射PCDB-hIGF-1组检测到392bp的特异性条带,注射PCDB组和对照组未检测到特异性条带;注射PCDB-hIGF-1组免疫组化均可见hIGF-1蛋白的阳性表达。结论hIGF-1基因在大鼠阴茎海绵体中有大量表达,PCDB质粒介导的海绵体内hIGF-1基因注射可部分恢复老龄大鼠的勃起功能。Objective To investigate the effects of intracavernosal injection of human insulin-like growth-1 (hIGF-1) gene on the erectile function in the aging rat. Methods 9-month-old male SD rats ( n= 35) were divided into 3 groups,the plasmid treated group was given a single intracavernosal injection of 100 μg PCDB-hIGF-1 and 150 μl PBS (n=15),another group was given a single intracavernasol injection of 100 μg pcDNA 3.1-myc-his B and 150 μl PBS (n=10).The control group was injected only 150 μl PBS in the cavernous...

关 键 词：勃起功能障碍 胰岛素样生长因子1 基因治疗 大鼠 

分 类 号：R698.1[医药卫生—泌尿科学]