银杏叶制剂对哮喘大鼠T淋巴细胞体外增殖和凋亡的影响  

Effects of Ginkgo Biloba Extract on Proliferation and Apoptosis of T Lymphocytes in vitro in Rats with Asthma

作　　者：唐以军[1] 徐永健[1] 张珍祥[1] 倪望[1] 陈仕新[1] 高宝安[1] 杜春玲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸内科,武汉430030

出　　处：《中国中西医结合杂志》2006年第S1期47-50,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的探讨银杏叶制剂治疗哮喘的部分机理。方法将14只大鼠分成2组(健康对照组7只,哮喘组7只),分别采集外周血,并分离出T淋巴细胞体外分组培养(分空白组、银杏叶制剂BN-52021组、银杏叶制剂EGb761组),各组分别以不同浓度的BN-52021和EGb761干预不同时间。用MTT法分别测定各组细胞的增殖情况,用Annexin V/PI双染法流式细胞仪测定BN-52021不同浓度组的细胞凋亡情况。结果与空白组比较,EGb761在低浓度时促进T淋巴细胞增殖,而高浓度时则起抑制作用(均P＜0．05)；BN- 52021对体外培养的哮喘和健康大鼠T淋巴细胞的增殖均有抑制作用,其作用强度(在一定的范围内)随剂量的增加和时间的延长而加强(均P＜0．05)；但对哮喘大鼠的抑制作用明显强于健康大鼠(P＜0．05)；T淋巴细胞凋亡率随BN-52021浓度增加而增加(P＜0．01)。结论银杏叶制剂因其成分和干预浓度的不同而对体外培养的大鼠T淋巴细胞增殖活性的影响不同,对哮喘与健康大鼠T淋巴细胞的作用也有差异。银杏内酯B是抑制T淋巴细胞增殖的主要活性成分,其不仅可抑制T淋巴细胞的体外增殖,而且还可增加T淋巴细胞凋亡率。Objective To explore the partial therapeutic mechanism of Ginkgo Biloba extract(GBE)in trea- ting asthma.Methods Fourteen SD rats were randomly divided into two groups,7 rats were sensitized as the asthmatic model group and the others taken as the healthy control group.T lymphocytes were isolated from periph- eral blood mononuclear cells(PBMCs)of the rats,and were cultured in vitro with Ginkgolide B(BN-52021 group)or Ginkgo Biloba extract 761(EGb761 group)in different concentrations or without any of them(c...

关 键 词：支气管哮喘 银杏叶提取物 T淋巴细胞 

分 类 号：R285[医药卫生—中药学]