常规细胞遗传学和荧光原位杂交方法检测14q32/IgH基因重排  

Conventional cytognetics and fluorescence in situ hybridization as methods for detecting IgH gene rearrangements

作　　者：代芸[1] 刘旭平[1] 李承文[1] 秦爽[1] 肖继刚[1] 黄琪[1] 徐方运[1] 贡金英[1] 王建祥[1] 刘世和[1] 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,血液病医院,天津300020

出　　处：《白血病．淋巴瘤》2006年第4期241-245,共5页Journal of Leukemia & Lymphoma

基　　金：天津市科技攻关计划资助项目(05YESZSF02400)

摘　　要：目的比较常规细胞遗传学(CC),间期荧光原位杂交(FISH)技术及连续R显带后FISH检测免疫球蛋白重链(IgH)基因重排的应用价值。方法应用常规细胞遗传学及间期FISH分析血液系统肿瘤患者43例。结果43例患者中14q32+/IgH+患者19例,14q32-/IgH+患者2例(4.7%),14q32+/IgH-患者3例(7.0%),14q32-/IgH-患者19例。部分病例CC与间期FISH方法检测14q32/IgH基因重排得到了不一致的结果。对5例患者进行连续R显带后FISH分析,对照核型和FISH图,能清楚地看到IgH基因易位涉及的染色体。结论间期FISH可以提高14q32/IgH重排检出率,R显带后FISH可以帮助识别与14q32易位的伙伴染色体。Objective The objectives of this study was to compare the value of conventional cytogenetics(CC), interphase FISH and sequential R-banding and FISH as methods for detecting IgH gene rearrangements. Methods 43 haemopoietic maligance patients were studied by CC and interphase FISH. Results Of the 43 patients,19 cases are 14q32+/IgH+, 2 cases are 14q32-/IgH+ (4.7 %), 3 cases are 14q32+/IgH- (7.0%) and 19 cases are 14q32-/IgH- in 43 patients. For some patients, we got different results by using CC and interphas...

关 键 词：细胞遗传学 荧光原位杂交 免疫球蛋白重链基因重排 

分 类 号：R55[医药卫生—血液循环系统疾病]