过氧化氢对黑素细胞生物学作用的研究  

Investigation on Biological Effects of H_2O_2 on Melanocytes

作　　者：谷梅[1] 柏志全[2] 肖瑞[3] 江振友[3] 

机构地区：[1]广东省皮肤性病防治中心,广东广州510500 [2]暨南大学医学院生理教研室,广东广州510600 [3]暨南大学医学院微生物教研室,广东广州510600

出　　处：《岭南皮肤性病科杂志》2006年第3期188-190,共3页Southern China Journal of Dermato-Venereology

基　　金：广东省医学科学技术研究基金(项目编号A2004144)

摘　　要：目的:研究不同浓度的过氧化氢(H2O2)对体外培养的正常人表皮黑素细胞的生物学作用。方法:分别以0.05、0.1、0.5、1 mmol/L的H2O2作用于体外培养的黑素细胞24 h,采用多巴氧化法测定酪氨酸酶活性;NaOH裂解法测定黑素合成;比色法测定丙二醛(MDA)含量。结果:正常人表皮黑素细胞经H2O2处理后,酪氨酸酶活性降低,黑素合成量减少,丙二醛含量增多,其中以0.5、1 mmol/L的H2O2作用明显,与对照组相比有统计学意义(P<0.05)。结论:H2O2在一定浓度时可抑制正常人表皮黑素细胞酪氨酸酶活性,减少黑素合成,并可致细胞过氧化损伤。提示H2O2在白癜风发病中可能起到一定作用。Objective:To investigate the effects of different concentrations of H_2O_2 on human epidermal melanocytes. Methods:Human epidermal melanocytes were cultured for 24h with 0.05、0.1、0.5、1 mmol/L H_2O_2 respectively.The tyrosinase activity was detected by DOPA-oxidation,the melanin content was measured by NaOH-assay,and MDA content was measured by its absorption at 532 nm.Results: H_2O_2 inhibited the tyrosinase activity,reduced melanogenesis and increased MDA content in the test groups between H_2O_2(0.5、1 mmol/L) and control,there is significant difference(P<0.05).Conclusion:H_2O_2 could inhibit the tyrosinase activity and reduce melanin contents of human epidermal melanocytes at a certain concentration,it was able to cause peroxidative injuries of cells.Maybe H_2O_2 play a role in the pathogenesis of vitiligo.

关 键 词：过氧化氢 黑素细胞 

分 类 号：R751[医药卫生—皮肤病学与性病学]