苜蓿雄性不育系组织培养技术的研究  

Studies on Tissue Culture Techniques of Male Sterile Line of Alfalfa

作　　者：高翠萍[1] 石凤翎[1] 孙静[1] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019

出　　处：《中国草地学报》2006年第5期29-32,共4页Chinese Journal of Grassland

基　　金：国家自然科学基金项目(30160057)

摘　　要：以苜蓿雄性不育系Ms-4幼嫩茎段、叶片、叶柄为外植体,诱导苜蓿雄性不育系再生植株。结果表明:叶柄是诱导愈伤组织的良好外植体,体细胞胚诱导率为90%,再生率49%。苜蓿雄性不育系插条生根的最佳处理为接种前用水进行预处理0.5h,外植体为完整插条,培养基为A1、A2,其生根率为97%,生根系数为5.3。The infancy stem segments,leaves and leafstalks of male sterile line Ms-4 of Medicago varia Martin.cv.Caoyuan No.1 were taken as explants and induced somatic embryos and roots of male sterile line Ms-4 of Medicago varia Martin.cv Caoyuan No.1.The results showed that leafstalks are good explants for inducing callus.Somatic embryos inducing rates was 90%,regeration rate was 49%.The best treatment of rooting of quickset of male sterile line Ms-4 of Medicago varia Martin.cv.Caoyuan No.1 is water treat for 0.5h before inoculabitity,integrity quickset,and A1 and A2 rooting medium,rooting rates was 97%,rooting coefficient was 5.3.

关 键 词：苜蓿 雄性不育系 组织培养 体细胞胚 

分 类 号：Q943.1[生物学—植物学]