内蒙古驼蹄瓣属植物亲缘关系的RAPD分析  

Studies on the Genetic Relationships of 7 Species of Zygophyllum in Inner Mongolia Based on Random Amplified Polymorphic DNA(RAPD)

作　　者：张辰波[1] 史雪松[1] 宛涛[1] 曹艳伟[1] 张晓明[2] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]海拉尔学院,内蒙古海拉尔025000

出　　处：《中国草地学报》2006年第5期86-90,共5页Chinese Journal of Grassland

基　　金：国家自然科学基金项目(30360070)

摘　　要：应用RAPD技术对七种驼蹄瓣属植物及其近缘种霸王的亲缘关系进行了研究,16个引物共扩增出125条带,其中99条表现出多态性,占79.2%,表现出丰富的RAPD多态性;利用UPGMA构建了分子系统树,结果表明RAPD分析的亲缘关系聚类基本上与经典分类相一致,表明RAPD技术适用于驼蹄瓣属植物亲缘关系的研究。Genetic relationship of 7 species of Zygophyllum L.and S.xanthoxylon Bunge were analysed by RAPD markers with 16 primers.125 bands were amplified,99(79.2%) of which were polymorphic.It showed that Zygophyllum has high genetic diversity.Molecular systematic tree constructed with UPGMA indicated that the results of clustering analysis based on RAPD was consistent with those based on traditional methods,which indicated that RAPD molecular marker could be used for genetic relationship researches on Zygophyllum.

关 键 词：驼蹄瓣属 RAPD 亲缘关系 霸王 

分 类 号：Q943[生物学—植物学]