酶催化合成L-半胱氨酸产酶培养基及产酶条件的研究  

Study on fermentation conditions and culture medium of enzymatic synthesis of L-cysteine

作　　者：寇广会[1] 周昌平[1] 刘淑云[1] 陈宁[1] 

机构地区：[1]天津科技大学天津市工业微生物重点实验室,天津300457

出　　处：《现代化工》2006年第z2期267-270,共4页Modern Chemical Industry

基　　金：天津市科委应用基础研究重点基金项目(05YFJZJC00901)

摘　　要：假单胞菌(Pseudomonas sp.)TS1138经DL-2-氨基-△2-噻唑啉-4-羧酸(DL-ATC)的诱导,在菌体内能产生催化该诱导物生成L-半胱氨酸的酶系.对产酶培养基及产酶条件进行了研究,得出DL-ATC的最适添加量为0.5%,最适碳源为葡萄糖,最适氮源为尿素,最适培养温度为27～29℃,最适摇瓶装液量为40mL.根据摇瓶优化结果,进行了7 L罐试验,酶活力最高达1 780 U/mL.L-cysteine synthesizing enzyme could be produced in Pseudomonas sp.TS1138,induced by DL-2-amino-Δ~2-thiazoline-4carboxylic acid(DL-ATC).Optimum conditions suitable for enzyme production in Pseudomonas sp.TS1138 were studied,and the results showed that enzymes activity in Pseudomonas sp.TS1138 was highest when the cultivate conditions were as follows:mass concentration of DL-ATC was 5 g/L,glucose and urea were carbon and nitrogen source respectively,fermentation temperature was 27-29℃,liquid volume of culture medium was 40 mL in a 500 mL shaking flask.The highest enzymes activity could reach 1 780 U/mL in a 7 L fermentor under the optimum conditions.

关 键 词：假单胞菌 DL-ATC L-半胱氨酸 

分 类 号：Q93[生物学—微生物学]