胶体金免疫层析法测定血清心肌型脂肪酸结合蛋白及临床应用  

Clinical application of gold immunochromatography assay of serum heart-type fatty acid-binding protein

作　　者：熊大迁[1] 余代美[1] 郭开均[1] 马韬[1] 

机构地区：[1]四川省成都中医药大学附属医院检验科,610072

出　　处：《检验医学与临床》2006年第3期100-101,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨国内研制的胶体金免疫层析法(GICA)试剂测定血清心肌型脂肪酸结合蛋白(HFABP)及其在急性心肌梗死(AMI)诊断中的应用价值。方法使用心肌型脂肪酸结合蛋白快速检测试剂盒检测血清HFABP。观察急性心肌梗死患者30例,在不同时间段检测HFABP、cTnI、CKMB,并与35例健康人检测结果比较。结果HFABP快速诊断试剂盒使用简单、方便、快速,手指血检测10～15min,血清检测只需3min。其检测灵敏度较高(6.5ng/ml),特异性较高。CKMB在AMI发生后0～4h阳性检出率极低,4～12h有一定的检出率,只有在12～24h有较高的阳性检出率;cTnI在6h内有较低的阳性检出率,在6h后检出率较高,维持时间较长。HFABP在AMI发生后0～4h即有较高的阳性检出率,4～12h维持高水平的检出率。结论胶体金免疫层析法(GICA)测定HFABP简便、快捷,灵敏度、特异性较高,适用于急诊检验。HFABP在AMI发生6h内较cTnI、CKMB对早期AMI更具诊断价值。Objective To estimate the value of gold immunochromatography assay(GICA) of serum heart-type fatty acid-binding protein for diagnosis of AMI.Methods H-FABP concentration of 35 healthy individuals and 30 AMI patients were measured by H-FABP reagent kit.cTnI,CK-MB were estimated in the same time.Results The reagent kit is rapid and simple.The test needed 10-15 min (or 3 min for serum).The sensitivity (6.5 ng/ml) and specificity are better.The detection rates of CK-MB was low in 0-4 h after attack of AMI,the lowest in 4-12 h,but higher in 12-24 h.The cTnI was detected with low deteotion rate at 6 h.The detection rate was high for long time after 6h.The H-FABP had high detection rate within 0-4 h and for 4-12 h after episode of AMI.Conclusion GICA is simple and rapid .The sensitivity and specificity are high.GICA is fit for emergercy .H-FABP has more diagnostic value than cTnI and CK-MB within 0-6 h after episode of AMI.

关 键 词：心肌型脂肪酸结合蛋白 心肌梗死 胶体金免疫层析法 

分 类 号：R446.61[医药卫生—诊断学]