纤维蛋白原B β-148(C→T)基因多态性与纤维蛋白原水平、颈动脉粥样硬化相关性研究  

To study relationship B βfibrinogene-148(C→T)gene polymorphism and plasma fibrinogene levels and carotid atherosclerosis

作　　者：刘瑛[1] 郭力[2] 张焕铃[3] 王志红[2] 王杏儒[1] 赵娜萍[1] 

机构地区：[1]石家庄市中心医院神经内科,河北石家庄050011 [2]河北医科大学附属第二医院,河北石家庄050000 [3]河北医科大学实验动物学部,河北石家庄050011

出　　处：《中风与神经疾病杂志》2006年第5期528-530,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究纤维蛋白原(Fg)Bβ-148(C→T)基因多态性与Fg水平、颈动脉粥样硬化相关性。方法用聚合酶链反应(PCR)限制性片段长度多态性(RFLP)分析方法;使用Clauss试剂盒采用自动检测法测定Fg水平;彩色多普勒诊断颈动脉粥样硬化并分级,0级<1mm,1级≤2mm,2级≤3mm,3级>3mm,4级完全闭塞。结果T/T型患者血浆Fg水平4.13±1.44与C/T型血浆Fg水平3.3±1.05、C/C型血浆Fg水平3.62±0.38比较,有显著性差异(P<0.05)。T/T型与严重的颈动脉粥样硬化明显相关(P<0.005)。结论FgBβ-148(C→T)突变基因T与Fg水平、严重的颈动脉粥样硬化明显相关。Objective To study relationship between B βfibrinogene-148(C→T)gene polymorphism and plasma fibrinogene levels and carotid atherosclerosis. Methods The B βfibrinogene-148(C→T)gene polymorphism of 77 individuals were analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The plasma fibrinogene levels was measured using Clauss reagent by the Keneticmethod. A color -coded device was used to determine atherosclerosis vessel wall of the carotid arteries. Carotid atherosclerosis was graded according to the most severe visible changes as follows:normal(0<1mm),1 grade≤2mm,2 grade≤3mm,3 grade>3mm,4 grade,completely bstructed. Results The three B βfibrinogene-148(C→T)genotypes were detected in 4 cases with C/C type,48 cases with C/T type and 25 cases with T/T type. There were also differences in fibrinogene levels among the C/C type,C/T and T/T B βfibrinogene-148(C→T)genotype (3.62±0.38g/L,3.3±1.05g/L,4.13±1.44g/L). The plasma fibrinogene level of T/T βfibrinogene genotype were significantly higher than in the other types(P< 0.05 ). Subjects carrying the T/T B βfibrinogene -148 genotype had higher grades of carotid atherosclerosis than the other two genotypes. Conclusion B βfibrinogene -148 T/T genotype is associated with fibrinogene levels and carotid atherosclerosis.

关 键 词：纤维蛋白原 基因多态性 颈动脉粥样硬化 

分 类 号：Q754[生物学—分子生物学]