醛固酮诱导扩张型心肌病小鼠心肌PCPE-1 mRNA的表达  

Expression of PCPE-1 mRNA in Murine Dilated Cardiomyopathy Induced by Aldosterone

作　　者：申锷[1] 陈瑞珍[1] 杨英珍[1] 郭棋[1] 虞勇[1] 邹云增[1] 陈灏珠[1] 

机构地区：[1]复旦大学附属中山医院上海市心血管病研究所卫生部病毒性心脏病重点实验室,上海200032

出　　处：《中国分子心脏病学杂志》2006年第5期268-271,封3,共5页Molecular Cardiology of China

基　　金：国家自然科学基金(30571741);国家博士点基金(20050246062)

摘　　要：目的探讨醛固酮是否影响柯萨奇病毒B_3(CVB_3)所致小鼠扩张型心肌病(DCM)心肌组织中PCPE-1(Procollagen C-Proteinase Enhancer 1)的表达。方法以CVB_3重复增量感染Balb/c小鼠建立病毒性扩张型心肌病动物模型(n=30)；小鼠感染CVB_3 24 h后以螺内酯(7．5 mg/kg/day)灌胃作为螺内酯干预(Spi)组(n=20)；同期腹腔无菌注射等容积不含病毒的EMEM液设立为正常对照(Con)组(n=10)。运用苦味酸天狼猩红胶原特异染色和偏光显微镜显像,并辅以图像分析软件计算心肌胶原容积积分(CVF)和Ⅰ型胶原的含量。应用RT-PCR技术检测心肌组织中Ⅰ型胶原(ColⅠ)和PCPE-1 mRNA的表达。结果DCM小鼠CVF和Ⅰ型胶原明显增多(P＜0．001),而这种增高的趋势在螺内酯干预组被明显遏制(P＜0．01)；与正常对照组比较,DCM小鼠心肌组织中PCPE-1及ColⅠmRNA同时异常增加(P＜0．001),螺内酯干预后PCPE-1及ColⅠmRNA的表达显著降低(分别为P＜0．01和P＜0．05)。结论醛固酮可刺激病毒感染导致的DCM小鼠纤维化心肌组织中PCPE-1 mR- NA的表达增高,PCPE-1将可能作为临床心肌纤维化药物干预潜在的作用靶点。Objective This study was designed to examine whether the expression of PCPE-1 mR- NA was affected by aldosterone in CVB_3-induced dilated myocardiopathy.Methods Balb/c mice were in- fected repeatedly with CVB_3 by increment to establish the experimental model of viral DCM mice(n=30). Spi mice(n=20)were intragastric administrated with spironolactone(7.5 mg/kg,d^(-1))after inoculated CVB_3 based above-mentioned methods and meanwhile control mice were treated with same volume EMEM without CVB_3.The changes of collagen in myocardium were stained with Sirius Red and observed by polar- imicroscope,CVF and collagen I were calculated assisted with image analysis software.The Col I and PCPE mRNA were determined by RT-PCR.Results The levels of both CVF and Col I in myoeardium of DCM mice increased markedly as compared to controls,an increase greatly reduced by spironolaetone.Corre- spondingly,the increase of mRNA in PCPE and Col I were observed in the hearts of DCM mice as compared to controls and were cut clown(P＜0.001)by spironolactone.Conclusions Aldosterone increased the ex- pression of PCPE mRNA in hearts from DCM mice induced with CVB_3,which suggests PCPE-1 as a new potential target for invention with cardiac fibrosis.

关 键 词：病毒 扩张型心肌病 纤维化 胶原 PCPE-1 

分 类 号：R542.2[医药卫生—心血管疾病]