载甲氨喋呤的磷酸钙骨水泥的力学性能测定及显微结构观察  

Biodynamics Properties and microstructrue observation of composite of Calcium phosphate cement and Methotrexate

作　　者：韩键[1] 杨志平[1] 李昕[1] 李建民[1] 

机构地区：[1]山东大学齐鲁医院骨外科,山东济南250012

出　　处：《生物骨科材料与临床研究》2006年第4期8-11,共4页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的研究加入不同浓度甲氨喋呤(MTX)对磷酸钙骨水泥(CPC)的凝固时间,力学强度及显微结构的影响,为进一步研制具有抗肿瘤复发作用的骨填充材料奠定基础。方法将甲氨喋呤(MTX)与磷酸钙骨水泥(CPC)分别以0．1%,0．2%,0．5%的质量比混合,并设立空白埘照,在预制模具中,制成直径0．8cm,高度1．2cm的圆柱体,将空白对照及上述不同浓度的CPC复合物分成A、B、C、D四个组,每组16个样本。测定其凝固时间,轴向压缩强度,横向压缩强度及旋转拉力强度,扫描电镜观察加入MTX前后CPC的显微结构变化。结果A、B、C、D各组的平均初凝及终凝时间分别随药物浓度增加而逐渐延长,但各组比较无统计学差异。B、C组各项平均轴向、横向压缩强度及旋转拉伸强度指标与A组比较得P值大于0．05,D组指标与A组比较P值小于0．05。扫描电镜可观察到CPC呈多孔晶体结构,加入甲氨喋呤不改变其基本晶体结构,MTX颗粒附着在CPC松针样结晶体表面或孔隙内。结论磷酸钙骨水泥(CPC)中加入适量甲氨喋呤(MTX),其基本晶体结构没有发生变化。加入适量不同浓度MTX CPC凝固时间无明显影响。质量比为0．1%、0．2%组与对照组力学强度无统计学差异,质量比为0．5%组CPC的力学强度与对照组有统计学差异,但其压缩强度位于松质骨与皮质骨之间,符合临床应用条件。Objective To explore the changes of setting time,mechanical properties and microstructrue of composite of various CPC-MTX,and to provide basic results of the bone filling materials that can suppress recur- rence of bone tumors.Methods Four MTX mass fractions are tested:0% (group A),0.1% (group B), 0.2% (group C),and 0.5% (group D),each group had 16 samples.The pasties of CPC-MTX were put in the prepared modular appliances and made into cylinder composites,to measure the axial direction compressive strength,transversal compressive strength and diametral tensile strength with CSS-2002 electronic universal ex- perimental machine;to observe the changes of CPC's microstructure with MTX comparing with conventional CPC without MTX.Results The setting time of c-CPC is prolonged when c-CPC contained increasing concentra- tion of MTX,but the setting times of B,C,D groups all have no significant difference compared with group A (P＞0.05).Microporous crystal structure of CPC and the diversities of microstructrue of c-CPC containing MTX can be distinctly observed with high resolution SEM Conclusions The microstructrue of c-CPC did not change when containing suitable MTX.But significant decreasing of index of 0.5% group can be observed compared with c-CPC,and there were no statistic significance of the other groups.However,the stress-bearing of group D is between cancelled bone and cortical bone and can be used in clinical application.

关 键 词：磷酸钙骨水泥 甲氨喋呤 凝固时间 生物力学测定 显微结构 

分 类 号：R318.08[医药卫生—生物医学工程]