银杏黄酮对卡铂所致大鼠肾损害的防护作用  

Preventive and Therapeutic Effect of Ginkgetin on Kidney Damage of Rat by Carboplatin

作　　者：屠晓钢[1] 王舒然[2] 乔凌[3] 

机构地区：[1]空后直供部卫生处,北京100035 [2]哈尔滨医科大学公卫学院营养与食品卫生教研室,哈尔滨150081 [3]空军大连通信士官学校门诊部,大连116600

出　　处：《中国实用医药》2006年第4期34-36,共3页China Practical Medicine

摘　　要：目的研究银杏黄酮(GBE)对卡铂(CBDCA)肾毒性的防护作用,并探讨其可能机制。方法灌胃给予大鼠GBE后腹腔内注射CBDCA,检测肾脏系数、血清尿素氮(BUN)及尿N-乙酰-β-D氨基葡萄糖苷酶(NAG)、血还原型谷胱甘肽(GSH)与肾皮质丙二醛(MDA)及线粒体谷胱甘肽过氧化酶(GSH-Px)及尿与肾皮质铂含量,观察GBE的防护作用的剂量依赖关系和经时过程。结果250、500和750mg/kgGBE预处理后第5天,CBDCA所致大鼠肾脏系数、NAG活性和BUN含量增高均不同程度减轻;500mg/kg组GBE预处理的效果最明显,该组肾脏系数、NAG活性和BUN含量分别为(0.79±0.12)g/100g、(15.86±3.28)U/gCre和(9.27±3.77)mmol/L,而CBDCA组这3项指标分别为(0.96±0.22)g/100g、(23.58±5.45)U/gCre和(31.08±15.00)mmol/L,上述各项指标两组间的差异有显著性(P<0.05或0.01)。GBE预处理可抑制CBDCA引起的MDA形成增高,GSH含量和GSH-Px活性下降,并能降低CBDCA所致大鼠肾皮质铂含量增高,促进铂经尿排泄。结论GBE有明显预防CBDCA的肾毒性,其部分机制为抗氧化作用,还可能与促进铂清除有关。Objective To research into preventive and therapeutic effect of ginkgetin(GBE) on kidney damage of rat by carboplatin(CBDCA) and investigate into possible mechanism.Methods After intragastric administration of GBE for rat, injected CBDCA into abdominal cavity , then detected kidney coefficient, serum urea nitrogen and urine N-acetyl-β-D-glucosaminidase(NAG), blood reduced glutathione, cortex renis malonaldehyde, bioblast glutathione peroxidase(GSH￡-Px) and platinum content of urine and cortex renis, observed dose-dependent relationship and time course of safety action of GBE.Results After 5 days of preconditioning by GBE of 250, 500 and 750mg/kg, increasing level of kidney coefficient, CBDCA, activity of NAG and content of BUN fell respectively. Effection of 500mg/kg GBE preconditioning was most obviously. This group has kidney coefficient[(0.79±0.12)g/100g], activity of NAG[(15.86±3.28)U/gCre] and content of BUN[(9.27±3.77)mmol/L]; but comparatively, CBDCA group has kidney coefficient [(0.96±0.22) g/100g], activity of NAG [(23.58±5.45)U/gCre], and content of BUN [(31.08±15.00)mmol/L]. Tow group have significantly difference (P<0.05 or 0.01). GBE preconditioning can restrain increasing of MDA and decreasing of content of GSH and activity of GSH￡-Px by CBDCA, at the same time, reduce increasing content of cortex renis of rat by CBDCA and promote homaluria of platinum.Conclusion GBE has obvious effect of prevention of CBDCA nephrotoxicity, partial mechanism is antioxidation; partial mechanism perhaps is connected with promoting clearance of platinum.

关 键 词：卡铂 银杏黄酮 肾毒性 抗氧化作用 

分 类 号：R96[医药卫生—药理学]