进出口植物产品中对硫磷残留酶联免疫检测试剂盒的研制  

Research on ELISA kit for parathion residues detection in importing and exporting plants

作　　者：周洪斌[1] 李平[1] 徐文久[1] 朱金连[1] 王勇涛[1] 方原民[1] 肖震[1] 

机构地区：[1]镇江出入境检验检疫局,江苏镇江212008

出　　处：《免疫学杂志》2006年第z1期182-185,共4页Immunological Journal

基　　金：国家科技部"十五"期间"食品安全关键技术"重大专项(2001BA804A17-8-03)

摘　　要：目的采用间接竞争ELISA法对进出口植物产品中对硫磷残留量进行快速筛选。方法将对硫磷还原成氨基对硫磷,利用适当的方法将其与牛血清白蛋白(BSA)进行偶联,制备免疫原免疫家兔并获得特异性抗体。结果采用传统的盐析法提取特异性免疫球蛋白(IgG)并建立了对硫磷间接竞争ELISA快速检测方法。抑制试验表明其检测范围在(100.0±1.56)ng/mL之间,可用于进出口植物产品中对硫磷的定量检测。该方法与气相色谱检测符合率为96%。样品的平均回收率为103%。交叉反应显示该抗体仅与对硫磷、氨基对硫磷反应,而与甲基对硫磷、马拉硫磷、杀螟硫磷、乐果等结构类似物几乎无交叉反应。结论本试剂盒具有操作简便、快速、灵敏等特点。Objective To screen parathion residues in importing and exporting plants through indirect competitive ELISA method.Methods Parathion was deoxidized to amido-parathion,and then was coupled to carrier proteins(BSA) as a antigen.This antigen was used to immunize the rabbits for specific antibody.Results IgG was extracted by traditional method [saturated(NH4)2SO4] and then the ELISA method for parathion was set up.The detection sensitivity was ranged from 1.56 ng/mL to 100.0 ng/mL,which is fit for the demand of quantitative determination of Parathion residue in plant productions.The coincidence rate of this method with the gas chromatography method was 96%.The average recovery of fortified samples was 103%.Cross-reactivity indicated that the antiserum could only react with parathion and amido-Parathion,but not with those substances whose structures are similar to parathion,such as parathion-Methyl,Malathion,Fenitrothion,or Dimethoate.Conclusion This testing kit is simple,rapid,and sensitive for detection of parathion residuces.

关 键 词：对硫磷 酶联免疫吸附试验(ELISA) 氨基对硫磷 

分 类 号：S41[农业科学—植物保护]