羊胎盘免疫调节活性因子(SPIF-Ⅰ)的分离纯化和鉴定研究  

Study on Purification and Characterization of Sheep Placental Immunoreglating Activity Factor-Ⅰ

作　　者：吴开平[1] 魏泓[1] 

机构地区：[1]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《天然产物研究与开发》2006年第5期796-798,832,共4页Natural Product Research and Development

基　　金：国家自然科学资金(30070120);重庆市2001年重点技术创新项目(147)

摘　　要：为探讨研究羊胎盘的活性组分,将羊胎盘绞碎通过匀浆、冻融、超虑等步骤制备羊胎盘提取液,经Sephadex G-50和Sephadex G-25凝胶过滤、Sephadex G-10凝胶柱脱盐,反相高效液相色谱纯化出单一组分并进行理化性质、生物活性初步研究。结果得到紫外特征吸收峰为273 nm、分子质量为303.8的羊胎盘免疫调节活性因子-Ⅰ(Sheep placental immunoreglating activity factor-Ⅰ,SPIF-Ⅰ)。体外生物学活性试验证明,SPIF-Ⅰ能显著影响小鼠脾淋巴细胞增殖分裂,具有明显的剂量-效应依赖关系。SPIF-Ⅰ是羊胎盘提取液中一种具有免疫调节的小分子活性多肽.The sheep placental immunoreglating activity factor-Ⅰ(SPIF-Ⅰ) that one of the components of sheep placenta was isolated and purified from sheep placenta extract by ultrafiltration,Sephadex G-50,Sephadex G-25 and Sephadex G-10 gel filtration chromatography.SPIF-Ⅰ exhibited a molecular mass of 303.8 by ESI-MS,and its abstract summit of ultraviolet ray was 273 nm.The results of biologicol activity showed that SPIF-Ⅰ specifically increased the proliferative respond of mitogen ConA-induced mouse splenocytes.That SPIF-I was a small chain peptide with immunoreglating activity of sheep placenta extract.

关 键 词：羊胎盘 凝胶层析 分离纯化 生物活性 

分 类 号：R284.2[医药卫生—中药学]