新型合成肽延长大鼠移植肾存活时间的实验研究  

Effects of a novel synthetic peptide on survival of rat renal allograft

作　　者：易善红[1] 

机构地区：[1]第三军医大学新桥医院泌尿外科,重庆400037

出　　处：《重庆医学》2006年第16期1453-1455,共3页Chongqing medicine

基　　金：国家新药基金资助项目(9690105184)

摘　　要：目的探讨一种新型合成肽对淋巴细胞免疫功能及大鼠移植肾脏存活时间的影响。方法采用人工标准固相合成法新型肽,3H-TdR掺入法观察其在体外对人外周单核细胞增殖反应的影响,建立原位大鼠异基因肾移植模型32例,设合成肽加CsA治疗组、合成肽治疗组、CsA治疗组以及对照组4组,每组8例,观察大鼠移植肾脏存活时间及移植肾功能。结果合成肽可显著抑制淋巴细胞转化及MLR的增殖反应;围手术期应用合成肽结合小剂量CsA,能显著延长同种大鼠移植肾脏的存活时间(54d),并维持较低的血清肌酐水平〔(47.40±11.2)mmol/L〕。结论合成肽能显著抑制人外周单核细胞经丝裂原或同种抗原刺激引起的增殖反应,围手术期应用新型合成肽结合小剂量CsA,能显著延长异基因移植物存活时间及功能,表明合成肽可能成为一种新型的器官移植免疫调节药物。Objectives To investigate the effects of a novel synthetic peptide(SP) on lymphocyte immune function and suvival of rat renal allograft.Methods Synthetic peptide was synthesized by Fmoc chemistry method.The effects of the peptide on alloreactive cytotoxic activities of human peripheral blood mononuclear cells were observed using 3H-TdR incorporation method. The model of orthotopic transplantation rat renal allograft into abbominal was established.Thirty-two F344 rats underwent unilateral nephrectomy and heterotopic transplantation with a LEW kidney.Group 1(8 cases) received no immunosuppression,group 2(8 cases) received subtherapeutic CsA alone,group 3(8 cases) received SP alone,group 4(8 cases) received subtherapeutic CsA +SP.The renal allograft survival days of receptor rats were observed.Serum creatinine was measured every week.Results The results showed that SP could obviously inhibit the proliferation of human peripheral blood mononuclear cells.Peptide therapy combined with a sub-therapeutic dose of CsA significantly prolonged allograft survival(54d),also reflected in significantly lower serum creatinine levels((47.40±11.2)mmol/L).Conclusion The novel synthetic peptide can obviously inhibit the proliferation of human peripheral blood mononuclear cells.Synthetic peptide therapy combined with a sub-therapeutic dose of CsA significantly prolonged allograft survival.The novel synthetic peptide can be adopted as a novel immunomodulatory drug to treat the transplantation rejection.

关 键 词：合成肽 淋巴细胞 移植 肾脏 大鼠 

分 类 号：R699[医药卫生—泌尿科学]