采用SCGE和SCE分析法研究姬松茸菌体多糖(Ab-Mp)对DNA损伤的抑制作用  

The protective effect of mycelium polysaccharides from Agaricus blazei(Ab-Mp)on damaged DNA

作　　者：孙亦阳[1] 杨兆芬[2] 谢继锋[1] 沈业寿[1] 

机构地区：[1]安徽大学生命科学学院,合肥230039 [2]福建师范大学生物工程学院,福州350007

出　　处：《生物学杂志》2006年第5期34-37,共4页Journal of Biology

基　　金：安徽省教育厅资助项目(2003jq103)

摘　　要：热水浸提法提取姬松茸菌丝体多糖,每天以10mg/(kg bw)和20mg/(kg bw)两种剂量对小鼠进行灌胃,连续15d。腹腔注射环磷酰胺(CP),运用单细胞凝胶电泳(SCGE)法和姐妹染色体交换(SCE)分析法,研究Ab-Mp对染色体损伤的保护作用。结果表明,Ab-Mp降低了CP损伤后彗星细胞的比例,缩短了彗星尾长;降低了CP损伤的姐妹染色单体交换率。Ab-Mp对环磷酰胺诱发的DNA损伤具有拮抗作用。A polysaccharide was isolated from mycelium of Agaricus blazei by hot water extraction.Ab-Mp was given at two levels,10mg/kg and 20mg/kg to male mice for 15 days by gavage,and then the mice received CP 40mg/kg by peritoneal injecting twice.The single cell gel-eleectrophoresis(SCGE)method and sister chromatid exchange(SCE)assays were employed to study the protective effect from Ab-Mp on the damaged chromosome.The results showed that Ab-Mp could decrease the frequencies of cells with tail comet and the tail lengths of liver cells.The frequencies of SCE were decreaed Ab-Mp had an anti-mutation effect on the DNA damage induced by cyclophosphqmide(CP)in mice.

关 键 词：姬松茸 单细胞凝胶电泳 DNA损伤 姐妹染色单体 抑制作用 

分 类 号：Q936[生物学—微生物学]