泰脂安胶囊对大鼠肝纤维化模型的影响  

Effect of Taizhi'an Capsules on the Model of Liver Fibrosis Induced by CCl_(4) in Rats

作　　者：童巧霞[1] 吴艳艳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院感染科,武汉430022

出　　处：《医药导报》2006年第10期991-994,共4页Herald of Medicine

摘　　要：目的探讨泰脂安胶囊对肝纤维化的治疗作用。方法用皮下注射四氯化碳的方法建立大鼠肝纤维化模型,治疗组大鼠在造模成功后灌胃给予0.1 g.mL-1泰脂安混悬液1 g.kg-1.d-1治疗8周。测定血清和肝脏匀浆丙二醛(MDA)、一氧化氮(NO)含量和一氧化氮合酶(NOS)的表达,VG染色检测肝脏组织中胶原纤维的表达,免疫组化检测肝组织转化生长因子β1(TGF-β1)、血小板源性生长因子(PDGF)和金属蛋白酶组织抑制因子1(TIMP-1)的表达。结果泰脂安能减轻肝纤维化的组织病理改变,降低肝纤维化大鼠肝组织胶原纤维和TIMP-1的表达。肝纤维化中,血清和肝脏匀浆MDA、NO含量和NOS的表达增加,肝脏组织TGF-β1、PDGF的表达增加,使用泰脂安可显著降低MDA、NO含量,抑制NOS、TGF-β1和PDGF的表达(P<0.01)。结论泰脂安对肝纤维化有治疗作用,其作用机制可能与影响MDA、NO含量及NOS、TGF-β1和PDGF的表达水平相关。Objective To probe into the effect of taizhi'an capsules on the model of liver fibrosis in rats. Methods 55 male SD rats were randomly divided into 3 groups: the control group(n=15),model group(n=20) and treatment group((n=20)).The model of liver fibrosis in rats of the model group and treatment group was established by subcutaneous injections of 3 mL·kg^(-1) of 40% CCl_(4) per rat twice a week for 12 consecutive weeks.After the establishment of the model,rats of the treatment group were given each 1 g·kg^(-1) of taizhi'an saline suspension(0.1 g·mL^(-1)) administered by gastrogavage q.d.for 8 consecutive weeks.Rats of the model group were given each equivalent volumes of 0.9 % sodium chloride solution via the same route as described.At the termination of the 8 week treatment course,blood samples and liver tissue were collected.Contents of malonyldialdehyde(MDA),nitric oxide(NO) and activities of nitric oxide synthase(NOS) in the serum and liver homogenate were determined.The level of collagenous fibers in the liver tissue was assayed with the Van Gieson staining.The expressions of the transforming growth factor beta 1(TGF-β1),platelet-derived growth factor(PDGF) and tissue inhibitor of metalloproteinase-1(TIMP-1) in the liver tissue were detected with immunohistochemiscal methods. Results Taizhi'an was shown to alleviate the histopathological changes of the liver and downregulate the expression of collagenous fibers and TIMP-1 in the liver tissue from rats with liver fibrosis.During the course of the liver fibrosis,the contents of MDA,NO and the expression of NOS in the serum and liver homogenate were increased and the expression of TGF-β1 and PDGF in the liver tissue was upregulated.In the present study,taizhi'an was shown to strikingly decrease the contents of MDA and NO,inhibit the expression of NOS,TGF-β1 and PDGF in the liver tissue(P<0.01). Conclusion Taizhi'an was shown to exert therapeutic effects on the liver fibrosis induced by CCl_(4) in rats,probably by decreasing the contents of MDA and NO,and

关 键 词：泰脂安 肝纤维化 一氧化氮 一氧化氮合酶 转化生长因子Β1 血小板源性生长因子 

分 类 号：R285.5[医药卫生—中药学]