驯鹿β-防御素-1(reBD-1)mRNA在驯鹿组织器官中的表达检测  

The examination of expression of Reindeer β-defensin reBD-1 mRNA in the tissues

作　　者：吴萨日娜[1] 赵艳芳[2] 余兴帮[3] 都格尔斯仁[1] 杨银凤[1] 

机构地区：[1]内蒙古农业大学动物胚胎与发育生物学研究室,呼和浩特010018 [2]呼伦贝尔市鄂温克旗农牧业局 [3]呼伦贝尔市兽医站

出　　处：《中国草食动物》2006年第6期23-26,共4页China Herbivores

基　　金：内蒙古农业大学博士启动基金(BJ04-2);内蒙古自然科学基金(200508010520)资助项目

摘　　要：为了检测reBD-1mRNA在驯鹿体内可能的表达器官,研究根据已知的reBD-1cDNA序列设计了一对预计扩增产物为121bp的引物,以驯鹿舌、食管、瘤胃、网胃、皱胃、十二指肠、回肠、结肠、肝、肺、气管、肾、膀胱、睾丸、附睾、心脏、脾脏中提取的总RNA为模板,采用反转录PCR(RT-PCR)技术检测驯鹿的上述器官内reBD-1mRNA的表达情况。结果显示reBD-1mRNA在上述组织器官中均有表达,其中在舌、瘤胃、睾丸中表达最强;在食管、十二指肠、结肠、气管、脾脏中有中等量的表达;在网胃、皱胃、回肠、肝、肺、肾、膀胱、附睾、心脏内的表达较弱。β-防御素reBD-1在驯鹿体内的广泛表达提示reBD-1有助于驯鹿的先天性宿主防御。In order to examine the organs that express possibly reBD-1 mRNA in a reindeer, we designed a pair of primers, which expected product was 121bp, according the known reBD-1 cDNA sequence. We extracted the total RNA from the tissues of a reindeer' s tongue , esophagus, rumen, reticulum, abomasum, duodenum, ileum, colon, liver, lung, trachea, kidney, bladder, testis, epididymis, heart, spleen and examined the expression of reBD-1 mRNA in above different tissues of the reindeer by RT-PCR. The result indicated that reBD-1 mRNA was expressed in the all organs that was examinated. The best expressed tissues of reBD-1 mRNA was tongue, rumen and testis; middle grade expressed tissues was oesophagus, duodenum, colon, trachea and spleen and in the reticulum, abomasums, ileum, liver, lung, kidney, bladder, epididymis and heart; the expression of reBD-1 mRNA was relatively low. The extensive expression of reBD-1 in reindeers suggests that reBD-1 may play a role in host defense.

关 键 词：Β-防御素 驯鹿 组织表达 

分 类 号：S825[农业科学—畜牧学]