重症急性胰腺炎大鼠胰腺组织中白血病抑制因子的表达变化  

Expression of Leukemia Inhibitory Factor in Pancreatic Tissues of Rats with Severe Acute Pancreatitis

作　　者：滕晓丽[1] 廖家智[1] 赵秋 杜静 谷华 覃华[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科

出　　处：《华中科技大学学报（医学版）》2006年第6期825-827,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省科技攻关计划资助项目(No.2002AA304B05)

摘　　要：目的观察重症急性胰腺炎(SAP)大鼠白血病抑制因子(LIF)在胰腺组织中表达的时相变化,探讨LIF在SAP病程中的意义。方法采用胰管逆行灌注5%牛磺胆酸钠的方法复制大鼠SAP模型。随机分为正常对照组(N组,n=6)、假手术组(Sham组,n=6)和重症急性胰腺炎组(SAP组,n=24)。用RT-PCR法检测胰腺组织中LIF mRNA的表达水平,免疫组织化学方法检测LIF在胰腺组织中的表达变化。结果N组和Sham组LIF表达呈阴性,SAP组LIF水平在建模后3 h已升高,至12 h和24 h仍维持在较高水平。结论LIF作为促炎症因子参与了SAP的炎症反应。Objective To observe the phase changes in the expression of leukemia inhibitory factor(LIF) in pancreatic tissues in rats with severe acute pancreatitis(SAP) and the significance.Methods SAP rat model was induced by retrograde injection of 5% sodium taurocholate into the pancreatobiliary duct.Animals were divided randomly into three groups: normal control(n=6),sham operation(n=6),SAP(subdivided into 3,6,12,24 h post-SAP subgroups,n=6 in each group).Intrapancreatic expression of LIF mRNA and protein was detected by semi-quantitative RT-PCR and immunohistochemistry respectively.Results The levels of LIF in pancreatic tissues were increased significantly at 3 h,and maintained at high levels up to 24 h after operation.Conclusion LIF seems to act as a proinflammatory factor in SAP.

关 键 词：急性胰腺炎 白血病抑制因子 白细胞介素-6 

分 类 号：R657.51[医药卫生—外科学]