某部驻区鼠类感染肾综合征出血热病毒的分子流行病学调查  

Survey on Molecular Epidemiology of Hantanviruse Carried by Rodents in a Military Base

作　　者：郝永建[1] 郭宝石[1] 邓致荣[1] 宋世佩[1] 郑金福[1] 白杨[1] 

机构地区：[1]第二炮兵防护防疫队,北京100071

出　　处：《实用预防医学》2006年第6期1459-1461,共3页Practical Preventive Medicine

摘　　要：目的了解某部驻区鼠类感染肾综合征出血热病毒状况和病毒型别。方法夹夜法捕鼠,计算鼠密度,确定鼠类的种群构成。应用逆转录-聚合酶链反应(RT-PCR)扩增鼠类脏器模板的M片段基因序列,用BLAST软件进行序列分析,并用Clustal X(1．8)进行聚类分析。结果共发现六种鼠类,分别为褐家鼠、大仓鼠、大林姬鼠、黑线姬鼠、棕背鼠平和花鼠,褐家鼠为优势鼠种,占35．85%；RT-PCR检测结果显示,2只大林姬鼠的肺和肝组织检测出了汉坦病毒,对一份阳性标本进行序列测定并分析其核苷酸序列,显示它为汉城型汉坦病毒。结论该部驻区鼠类携带汉城型汉坦病毒,主要宿主动物为大林姬鼠。Objective To investigate hantanvirus infection of captured rodents in a military base and to type hantanvirus by using molecular technique.Methods The captured mice were classified and the density was calculated.Reverse tran- scription- polymerase chain reaction(RT-PCR)technique was used to amplify the partial M fragments of hantanvirus.A representative positive sample was sequenced and analyzed by Genbank BLUST and Clustal X(1.8) software.Results Six species of rodents(Batttus norvegicus,Cricetulus trition,Apodemus specious,Apodemus agrarius,Clethrionomys rutius and Eutanmias sibirion) were captured,among which Batttus norvegicus was the dominant group,accounted for 35.85 %.The hantanvirus was detected from the organs of Apodemus specious.Nucleotide sequences analysis showed that the virus detect- ed belongs to the SEO-type.Conclusion The SEO-type of hantanviruse was carried by rodents in a military base,the major host of hantanvirus was Apodemus specious.

关 键 词：肾综合征出血热病毒 分子流行病学 序列分析 

分 类 号：R373.3+2[医药卫生—病原生物学]