高效液相色谱法测定苦玄参药材中的苦玄参苷ⅠA含量  

Determination of Piefeltarraenin Ⅰ A Extracted from Picria fel-terrae Lour. by HPLC

作　　者：甄汉深[1] 何翠薇[1] 陈勇[1] 周吴萍[1] 苏春妹[1] 陆晖[1] 

机构地区：[1]广西中医学院,广西南宁530001

出　　处：《广西科学院学报》2006年第z1期403-405,共3页Journal of Guangxi Academy of Sciences

基　　金：国家中医药管理局课题民族项目(04-05ZM01);广西科技厅攻关项目(桂科攻0235022-4);广西教育厅课题(桂教科研[2003]22号)

摘　　要：采用Agilent ZORBAX Eclipse XDB C8 色谱柱(4.6×150mm,5μm),以乙腈-0.5%醋酸(32:68)为流动相,流速1ml·min-1,检测波长264nm,建立高效液相色谱法(HPLC),测定广西梧州大坡种植基地和龙州县种植的苦玄参药材的茎、叶中苦玄参苷ⅠA的含量.实验结果显示,苦玄参苷ⅠA在2.28～11.4μg范围内线性良好,平均加样回收率为101.1%RSD=2.4%(n=5),梧州大坡种植基地和龙州县种植的苦玄参药材中苦玄参苷ⅠA在茎、叶中的平均含量分别为0.10%,0.31%,0.11%,0.58%.本方法简便、快速,重复性好,可实际用于苦玄参药材中苦玄参苷ⅠA含量测定.Using the Agilent ZORBAX Eclipse XDS C_8 column(4.6×150mm,5μm),with the mobile phase acetonitrile-0.5% acid(32:68),determined by 264nm ultraviolet,1ml/min flow rate,to quantify the main constituent Piefeltrraenin ⅠA in the stems and leaves of Picria fel-terrae Lour.,which were planted in Wuzhou,Dapo and Longsheng of Guangxi.The result showed that linearity of Piefeltarraenin ⅠA was good in the range of 2.28~11.4μg,the recoveries of Piefeltarraenin ⅠA were 101.1%,respectively with corresponding RSD=2.4%(n=5).The main constituent Piefeltarraenin ⅠA in Picria fel-terrae Lour.in two places were 0.10%,0.31%,0.11%,0.58%.The HPLC method has the characteristics of time saving,convenience,good repeatability which can be used for quantifying the main constituent Piefeltarraenin ⅠA in Picria fel-terrae Lour.

关 键 词：高效液相色谱法 苦玄参 苦玄参苷ⅠA 含量测定 

分 类 号：O657.72[理学—分析化学] R284[理学—化学]