HPLC法测定山绿茶不同炮制品种中绿原酸和芦丁的含量  

Content Determination of Chlorogenic Acid and Rutin in Different Processed Shanlücha by HPLC

作　　者：陈勇[1] 李艳苓[1] 李立[1] 潘丽娜[1] 李兵[1] 谢臻[1] 

机构地区：[1]广西中医学院药学院,广西南宁530001

出　　处：《广西科学院学报》2006年第z1期416-418,421,共4页Journal of Guangxi Academy of Sciences

基　　金：广西科技厅科技攻关项目(合同号:桂科攻0322024-4B)

摘　　要：为了选出山绿茶的最佳炮制工艺,建立山绿茶中绿原酸和芦丁的含量测定方法,采用高效液相色谱法(HPLC)测定山绿茶不同炮制品中绿原酸和芦丁的含量.结果表明,HPLC法能较好地测定山绿茶中绿原酸和芦丁的含量,绿原酸进样量在0.01032～0.6192μg范围内有良好的线形关系,r=0.9998,平均回收率98.86%,RSD为2.54%.芦丁进样量在0.02004～1.2024μg范围内有良好的线形关系,r=0.9999,平均回收率102.31%,RSD为2.17%.该法简便、准确、专属性强,可作为检测山绿茶中绿原酸和芦丁含量的方法.To select the best processing method for shanlücha and establish a method for content determination of chlorogenic acid and rutin in shanlücha.Using HPLC to determine the contents of chlorogenic acid and rutin in shanlücha.Chlorogenic acid and rutin can be determined by HPLC,the chlorogenic acid showed a good linear relationship in a range of 0.01032～0.6192μg,r=0.9998,the average recovery was 98.86% and RSD was 2.54%,and the rutin also showed a good linear relationship in a range of 0.02004～1.2024,r=0.9999,the average recovery was 102.31% and RSD was 2.17%.The method is simple,accurate,with strong specificity,which can be used for the quantitative determination and control of shanlücha.

关 键 词：高效液相色谱法 绿原酸 芦丁 山绿茶 

分 类 号：O657.72[理学—分析化学]