秦艽龙胆苦苷在家兔体内的药代动力学研究(英文)  

PHARMACOKINETICS STUDY OF GENTIOPICROSIDE IN GENTIANA IN RABBITS

作　　者：李向阳[1] 李福安[2] 冯伟力[1] 袁明[2] 尼玛才让[3] 朱俊博[1] 

机构地区：[1]青海大学医学院药学系 [2]青海大学医学院中医系 [3]青海藏医院

出　　处：《青海医学院学报》2006年第4期227-230,247,共5页Journal of Qinghai Medical College

基　　金：The project was supported by the Youth Foundation of Qinghai University Medical College(04-001)and the Natural Science Foundation of Qing-hai Province of China(2001-N-107-03)

摘　　要：目的 建立家兔血浆中龙胆苦苷的RP-HPLC测定法.方法 血样经乙腈预处理后,进行HPLC分析,色谱柱为Lichrospher C18 (5 mm,250 mm×4.6 mm),流动相为甲醇-水-冰乙酸-三乙胺 (30:70:0.2:0.3, v/v).用秦艽提取液给10只健康家兔灌胃,计算主要药动学参数.结果 在1～ 60 μg/ml范围内龙胆苦苷浓度与峰面积线性关系良好(r = 0.9996), 定量限为1 μg/ml,日内、日间精密度均小于10%,回收率为86.4%～92.3%.龙胆苦苷在家兔的主要药代动力学参数tmax 、Cmax 、t1/2 和AUC分别为1.35 h、31.29 μg/ml、1.19 h和99.5 μg·h/ml.结论 所建分析方法灵敏、准确、简便,可作为秦艽龙胆苦苷的药代动力学研究方法.objective A highly sensitive method for quantitation of gentiopicroside in rabbit plasma was established by using high performance liquid chromatography.Methods After got from 10 rabbits,plasma were treated by acetonitrile and separated by HPLC on a C_18 reversed-phase column using mobile phase of methanol-water-glacial acetic acid-triethylamine(30:70:0.2:0.3,v/v).Results Calibration curves,which were linear over the range of 1 to 60 μg/ml,were analyzed contemporaneously with each batch of samples,along with low(1 μg/ml),medium(10 μg/ml) and high(60 μg/ml) quality control samples.The intra and inter-assay variability ranged from 3.22 to 7.33% for the low,medium and high quality control samples.The absolute recovery of gentiopicroside from plasma was in the range of 86.4-92.3%.Pharmacokinetics parameters of gentiopicroside were as follow: t_max and C_max were 1.35 h and 31.29 μg/ml respectively,t_1/2 was 1.19 h,and AUC was 99.5 μg·h/ml.Conclusion The method was sensitive,accurate and simple for the determination of gentiopicroside in rabbit plasma and it has been used successfully to study gentiopicroside pharmacokinetics in gentiana in adult rabbits.

关 键 词：秦艽 龙胆苦苷 药代动力学 高效液相色谱 

分 类 号：Q949.776.4[生物学—植物学]