清脂胶囊对内毒素诱导的炎症介质水平的影响及时效关系  

Inhibition Effects of Qingzhi Capsule on Inflammatory Reaction Attacked by LPS

作　　者：赵桂芝[1] 聂淑琴[1] 杨庆[1] 翁小刚[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2006年第11期39-42,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究清脂胶囊对饲喂高脂饲料及注射内毒素诱导的炎症小鼠模型炎症介质及血管活性物质水平的影响及经时变化。方法:小鼠喂饲高脂饲料的同时给药10d后腹腔注射内毒素(LPS)360μg/kg,注射LPS后分别于2,4,6h眶血管丛取血测C反应蛋白(CRP),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α)含量。注射LPS后2,6h取抗凝血测血栓素B2(TXB2),6-酮-前列腺素F1α(6-keto-PGF1α)含量。结果:在实验设定的各个时间点,注射LPS后模型组中TXB2,6-keto-PGF1α,CRP,IL-6,TNF-α水平较正常组均有明显升高;清脂胶囊可以明显降低LPS注射后2h和6h血浆中TXB2和6-keto-PGF1α水平,改善6h TXB2/6-Keto-PGF1α比值。明显降低模型小鼠LPS注射后6h血清中IL-6,4h和6h血清中CRP的含量。结论:清脂胶囊主要通过抑制花生四烯酸代谢途径生成的TXB2及6-keto-PGF1α抑制内毒素诱导的炎症反应。Objective:To investigate the influence of Qingzhi Capsule on mediators of inflammation and vasoactive substances in the model of LPS attack with high fat diet in mice.Methods:With high fat diet and oral qingzhi capsule for 10 days,then induced by ip.LPS at the last day.The blood was collected immediately from ophthalmovascula clump,at 2,4,6h.To measure the level of CRP,IL-6 and TNF-α.At 2h and 6h the blood of mice was collected immediately from ophthalmovascula clump,To measure the level of plasma TXB_2 and 6-k-PGF_(1α).Results:After ip.LPS in each time point,the level of CRP,IL-6,TNF-α,TXB_2 and 6-K-PGF_(1α) in model group have Obvious difference from the normal group;Qingzhi Capsule had strong effect on prevention of increasing TXB_2 and 6-K-PGF_(1α),Results also indicated depressing of TXB_2/6-K-PGF_(1α) ratio in the mice.Qingzhi Capsule depressed the level of CRP at 4h and 6h in the mice with high fat diet and LPS attack,depressed the levels of IL-6 at 6h.Conclusion:Qingzhi Capsule inhibited the expression of inflammatory factors in the mice with high fat diet and LPS attack.

关 键 词：清脂胶囊 内毒素损伤 C反应蛋白 肿瘤坏死因子-α 白介素-6 血栓素B2 6-酮-前列腺素F1Α 

分 类 号：R285.5[医药卫生—中药学]