春花胡枝子黄酮大孔树脂纯化工艺研究及纯化前后抗氧化性变化比较  

Researches on Purification Technologic Conditions of Lespedeza dunnii Flavonoids with AB-8 Macroporous Resin and Comparision of their Purification Antioxidant Changes before and after Being Purified

作　　者：周守标[1] 陈乃东[1] 胡金蓉[1] 罗琦[1] 王贵军[1] 王春景[1] 

机构地区：[1]安徽师范大学生命科学学院重要生物资源保护与利用安徽省重点实验室,安徽芜湖241000

出　　处：《中国实验方剂学杂志》2006年第12期2-7,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：安徽省自然科学基金(NO:00042415);安徽省重要生物资源保护与利用重点实验室基金;安徽省教育厅自然科学基金重点项目(NO:2006KJ060A)

摘　　要：目的:研究AB-8型大孔吸附树脂纯化春花胡枝子总黄酮的条件、参数及纯化前后体外抗氧化能力的变化.方法:通过静态实验、动态实验对影响粗黄酮纯化的因素进行研究;同时,对春花胡枝子黄酮的体外抗氧化能力进行测定.结果:吸附原液浓度为0.3 mg/mL;静态吸附时,温度20～40℃,pH=4～5条件下吸附,并以70%的乙醇作为解吸剂效果较好;动态时,pH=4～5,以0.5～1.0 mL/min流速上样,70%～90%的乙醇以0.5～1.5 mL/min洗脱效果较好.浓度0.12 mg/mL的精制黄酮和粗黄酮对羟自由基清除率分别为78.43%和29.25%;浓度0.375 mg/mL的精制黄酮和粗黄酮对邻苯三酚自氧化反应速度的抑制率分别为98.49%及63.02%.结论:AB-8型大孔吸附树脂对春花胡枝子粗黄酮纯化效果较好,纯度及体外抗氧化能力都得到较大提高.Objective: To study the conditions and parameters of purifying total flavonoids from L.dunnii with AB-8 macroporous resin and to compare with antioxidant ability before and after being purified.Method:Some factors which effected purification in static and dynamic adsorptions were studied.Furthermore,the antioxidant ability were also determined.Result:The concentration of the sample solution was 0.3 mg/mL.In static adsorption,the effects were preferable when temperature was at 20～40 ℃ and pH value was 4～5 and using 70% ethanol as desorption reagent. In dynamic adsorption,pH value of sample solution was 4～5 and flowing rate of loading was 0.5～1.0 mL/min. Using 70%～90% ethanol and 0.5～1.5 mL/min flow rate,the eluting effect was good.The clearance rates of 0.12 mg/mL refined flavonoid powder and crude flavonoid powder were 78.43% and 29.25% respectively.The inhibitory rates to pyrogallol autoxidation of 0.375 mg/mL crude flavonoid powde and refined flavonoid powder were 98.49% and 63.02% respectively.Conclusion:AB-8 macroporous resin had an excellent purification effect to crude flavonoid of Lespeda dunnii.The purity and antioxidant abbility were improved remarkably.

关 键 词：春花胡枝子黄酮 AB-8型大孔吸附树脂 静态吸附 动态吸附 清除率 抑制率 

分 类 号：R286.3[医药卫生—中药学] R285.5[医药卫生—中医学]