一氧化氮合酶对大黄游离蒽醌提取物致小鼠腹泻的影响  

Anthraquinone extractive from Rheum Palmatum L.induced diarrhea through increasing the activities of NOS in mice

作　　者：唐大轩[1] 罗培[2] 程蕾[1] 谭正怀[1] 梁媛媛[1] 

机构地区：[1]四川省中医药科学院,四川成都610041 [2]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2008年第3期271-273,共3页West China Journal of Pharmaceutical Sciences

基　　金：四川省科技厅应用基础项目资助(04JY029-005-7)

摘　　要：目的研究大黄游离蒽醌的致泻作用及与NO的相关性。方法分别测定小鼠在给药后的排稀便时间和次数;在给小鼠预防性ip 40 mg.kg-1NO合酶抑制剂NAME后30 min,分别ig不同剂量的大黄游离蒽醌或等容量蒸馏水,30 min后处死小鼠,分别测定其胃、小肠及结肠内容物的重量和总蛋白,以及小肠和结肠内容物中K+、Na+、Cl-的含量。结果100、200 mg.kg-1游离蒽醌均可引起小鼠腹泻。L-NAME可显著对抗游离蒽醌致小鼠腹泻及增加小鼠胃、小肠及结肠的内容物、蛋白质浓度,调节电解质水平。结论大黄游离蒽醌的致泻作用与NO合酶通路的活性改变有关。OBJCTIVE To study the relation between NOS and the effect of anthraquinone from Rheum Palmatum L.(AERP) induced diarrhea in mice.METHODS To evaluate the effects of AERP inducing diarrhea in mice,the time of starting water feces and the times of water feces in 3 hours after given AERP were recorded.To study the effects of NOS on diarrhea induced by AERP,the mice were ip N(G)-nitro-L-arginine-methyl ester(NAME)40 mg·kg-1 30 min before ig AERP,and then were killed 30 min later.The contents in stomach,small int...

关 键 词：大黄游离蒽醌 L-NAME 致泻 一氧化氮合酶 

分 类 号：R96[医药卫生—药理学]