尿苷产生菌的选育  

The Breeding of Uridine Producing Strain

作　　者：程远超[1] 刘康乐[1] 徐庆阳[1] 

机构地区：[1]天津科技大学生物工程学院,天津300222

出　　处：《现代食品科技》2007年第1期20-22,共3页Modern Food Science and Technology

摘　　要：以野生型枯草芽孢杆菌(Bacillussubtilis)TJ374为出发菌株,采用DES(硫酸二乙酯)及UV(紫外)诱变处理,筛选具有2TUr(2-硫尿嘧啶抗性)和6AUr(6-氮尿嘧啶抗性)及UP-(尿苷磷酸化酶缺失)标记的菌株,获得一株尿苷产生菌Tc142,36℃摇瓶发酵72h最高可产尿苷3.34g·L-1。A mutant Bacillus subtilis Tc142 producing uridine was obtained by DES and subsequent UV mutagenesis using wild type Bacillus subtilis TJ174 as the parent strain. The mutant Tc142 has been marked with 2 TUr、6 AUr and UP, using which, uridine content in the fermentive liquor at 36℃ after 72 h reached 3.34g/L.

关 键 词：枯草芽孢杆菌 尿苷 菌种选育 

分 类 号：TQ464[化学工程—制药化工]