N-乙酰-L-半胱氨酸溶液氧化反应速率常数的测定  

Determination of oxidation rate constant of N-acetyl-L-cysteine in aqueous solution

作　　者：付茂琦[1] 李琳丽[1] 詹先成[1] 齐海军[1] 何宁[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2008年第4期452-454,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金(No.30472104)

摘　　要：目的测定N-乙酰-L-半胱氨酸(NAC)在水溶液中氧化反应的速率常数。方法在一定温度下,向反应液中持续通入空气,以维持溶解氧浓度恒定,利用氧电极测定氧的浓度;采用碘量法测定NAC在水溶液中不同时刻的浓度,计算氧化反应降解速率常数。结果试验温度下,在pH9.0、pH6.8的缓冲溶液中,NAC与氧的反应均为表观零级反应,298.2 K时平均表观反应速率常数分别为8.03×10-3、0.08 mmol.L-.1h-1;而在pH4.0的缓冲溶液中,NAC与氧的反应为表观一级反应,298.2 K时,平均表观反应速率常数为0.02 h-1。结论弱碱性和中性条件下反应速率较慢,NAC的抗氧化能力较强;在弱酸性条件下反应速率较快,抗氧化能力较弱。在NAC溶液的初始浓度及pH相同条件下,温度升高,表观反应速率常数增大。OBJECTIVE To determine the oxidation reaction rate constant of N-acetyl-L-cysteine(NAC) in aqueous solution.METHODS A sufficient air was inflated into the solution continually to keep the concentration of dissolved oxygen steady.The concentration of dissolved oxygen was determined by electric potential method.The residual concentration of NAC in the solution was measured with iodimetry.The reaction rate constant could be obtained from a linear regression method.RESULTS In weak alkaline and neutral buffer so...

关 键 词：N-乙酰-L-半胱氨酸 氧 氧化反应速率常数 测氧仪 碘量法 

分 类 号：R927[医药卫生—药学]