浊度法测定硫酸妥布霉素注射液的含量  

Determination of Tobramycin sulfate injection by turbidimetric assay method

作　　者：湛文青[1] 彭洁[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《华西药学杂志》2008年第4期494-495,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用浊度法测定硫酸妥布霉素注射液的含量。方法选用金黄色葡萄球菌[CMCC(B)26 003]为试验菌,利用妥布霉素在含菌液体培养基中,于530 nm的吸光度与浓度的对数呈良好线性关系的原理进行测定。结果妥布霉素0.3~1.1μg.ml-1线性关系良好(r=0.9977),平均回收率为102.3%,RSD=1.6%(n=9)。结论所建方法较常用的管碟法操作简便快速、灵敏、准确、稳定,是测定硫酸妥布霉素注射液含量的较好方法。OBJECTIVE To establish a turbidimetric assay method to determine Tobramycin sulfate injection.METHODS The test bacteria was staphylococcus aureus [CMCC(B)26 003];A good linear relationship between the logarithm of concentration and its absorbance was obtained at the wavelength of 530 nm when tobramycin was in the liquid medium containing the test bacteria.RESULTS The standard curve was linear over the range of 0.3-1.1 μg·ml-1(r=0.9977).The average recovery was 102.4% with RSD of 1.6%(n=9).CONCLUSION The met...

关 键 词：浊度法 硫酸妥布霉素注射液 妥布霉素 含量测定 

分 类 号：R927[医药卫生—药学]