HPLC同时测定乐脉颗粒中的丹酚酸B和丹参素钠  

Determination of salvianolic acid B and sodium danshensu in Lemai granule by HPLC

作　　者：王欣眉[1] 蒋慧[2] 徐小平[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川省第四人民医院药剂科,四川成都610016

出　　处：《华西药学杂志》2008年第4期476-477,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立同时测定乐脉颗粒中丹酚酸B和丹参素钠含量的方法。方法采用HPLC法,色谱柱为Shim-pack C18柱(150 mm×4.6 mm,5μm),流动相A为甲醇,B为5%冰醋酸溶液,梯度洗脱。流速1.0 ml.min-1;检测波长286 nm;柱温35℃。结果丹酚酸B和丹参素钠分别在0.1933~2.8995、0.0807~1.2105μg线性关系良好,r均为0.9999,丹酚酸B和丹参素钠的平均回收率分别为98.54%、98.36%,RSD分别为0.69%、0.57%(n=6)。结论所建方法操作简便、结果准确,重复性好,可用于该制剂的质量控制。OBJECTIVE To establish a method for the determination of salvianolic acid B and sodium danshensu in Lemai granule.METHODS An HPLC method was used.The Shim-pack ODS column(150 mm×4.6 mm,5 μm) was used at the temperature of 35℃.The mobile phase consisted of A:methanol,B:5% acetic acidglacial.The flow rate was 1.0 ml·min-1 and the detective wavelength was at 286 nm.RESULTS The linear ranges of salvianolic acid B and sodium danshensu concentration were from 0.1933 to 2.8995 μg and from 0.0807 to 1.2105 μg.The c...

关 键 词：乐脉颗粒 丹酚酸B 丹参素钠 HPLC 

分 类 号：R286.0[医药卫生—中药学]