三尖杉愈伤组织诱导及其分化的初步研究  

Preliminary study on callus induction and differentiation of Cephalotaxus fortunei

作　　者：蔡月琴[1] 林镇斌 陈礼光[3] 刘学琴[3] 付影[3] 郑郁善[3] 

机构地区：[1]漳州师范学院生物科学与技术系,福建漳州363000 [2]三明市林业局,福建三明365001 [3]福建农林大学林学院,福建福州350002

出　　处：《福建林学院学报》2008年第1期31-35,共5页Journal of Fujian College of Forestry

基　　金：福建省林木种苗科技攻关计划项目(021812)

摘　　要：以三尖杉茎尖、嫩叶为外植体,应用正交设计法对愈伤组织诱导及其继代分化进行了研究。结果表明,不同外植体灭菌的难易程度相差较大,叶片较茎尖容易;叶片用体积分数为75%的酒精浸泡30-45 s,经体积分数为0.1%HgCl2溶液灭菌12 min,效果最好;茎尖则用体积分数为75%酒精浸泡1 min,经体积分数为0.1%HgCl2溶液灭菌12 min,效果较好;诱导叶片、茎尖愈伤组织的最佳培养基配方为MS+NAA 1.0 mg.L-1+2,4-D 1.0 mg.L-1;愈伤组织在继代培养基上均能进行分化,其中结构紧密的茎尖愈伤组织在MS+6-BA3.0 mg.L-1培养基上继代培养能诱导分化形成不定芽。With the orthogonal design,the young stem tip and tender leaf of Cephalotaxus fortunei Hook.f.were used for tissue culture and the induction and differentiation of callus.The results indicated that there was difference between sterilization of explants,the tender leaf was disinfected easily.The best sterilization procedure for the tender leaf was 0.1%HgCl2 for 12 minutes after 75% alcohol for 30-45 seconds and the best sterilization procedure for the stem tip was 0.1%HgCl2 for 12 minutes after 75% alcohol f...

关 键 词：三尖杉 愈伤组织 诱导 继代培养 

分 类 号：S791.48[农业科学—林木遗传育种]