粉防已碱对戊四唑致癫大鼠脑透析液中Glu、Asp的影响  

The effects of tetradrine on the extracellular levels of Glu and Asp in the Pentylentetrazol-induced rats

作　　者：刘学文[1] 金英[2] 王倩[3] 郎森阳[1] 

机构地区：[1]解放军总医院神经内科,北京100853 [2]辽宁医学院药理学教研室 [3]辽宁医学院附属第一医院放射科

出　　处：《脑与神经疾病杂志》2008年第3期185-187,共3页Journal of Brain and Nervous Diseases

摘　　要：目的:探讨钙拮抗剂粉防已碱(Tet)的抗癫痫作用机制。方法:健康SD大鼠随机分为4组:对照组、Tet组3个剂量组(15mg/kg、30mg/kg、60mg/kg)。将大鼠制成微透析模型,并收取其腹腔注射戊四唑(Pentylenetetrazol,PTZ)前后的透析液,采用高效液相色谱和荧光检测相结合的方法测定大鼠海马细胞外谷氨酸(Glu)及天冬氨酸(Asp)的浓度。结果:对照组腹腔注射PTZ后海马细胞外Glu浓度明显升高(p<0.01),Tet三个剂量组腹腔注射PTZ后同对照组比较Glu、Asp浓度明显降低(p<0.01),且似具剂量依赖性。结论:Tet的抗癫痫作用与其降低癫痫大鼠海马细胞外Glu及Asp浓度有关,对Glu的作用更为显著。Objective:To observe the mechanisms of Tetradrine on the model of PTZ-induced seizures in rats.Methods:The in vivo microdialysis and HPLC technique were utilized to measure the extracellular levels of glutamate and aspartic acid in the hippocampus in rats consecutively after given PTZ or pre-administrated Tetradrine.Results:The experimental seizure was mitigated compared with control groups.The hippocampus extracellular glutamate and aspartic acid level increased significantly in all rats which were not giv...

关 键 词：癫痫 粉防己碱 谷氨酸 天冬氨酸 

分 类 号：R285.5[医药卫生—中药学]