三种方法测定血小板及其各参数性能的比较  

Evaluation of two-dimensional platelet analysis on the ADVIA120 analyzer

作　　者：陆学军[1] 高洁[1] 吴巍[1] 

机构地区：[1]卫生部北京医院检验科,北京100730

出　　处：《检验医学与临床》2005年第6期247-248,254,共3页Laboratory Medicine and Clinic

摘　　要：目的激光法与手工法及电阻抗法测定血小板进行比较,并统计计算激光法测定血小板时血小板计数(PLT)、平均血小板体积(MPV)、血小板压积(PCT)、血小板分布宽度(PDW)、平均血小板内容物浓度(MPC)、平均血小板内容物含量(MPM)和大血小板(LPLT)的正常参考范围。方法随机抽取117例患者静脉血样本,分别用手工法、ADVIA120和CD3500进行血小扳计数;抽取1002例正常体检人员静脉血进行血小板及各参数的测定。结果手工法与ADVIA120计数血小板无显著差别(P>0.05),相关性较好(r=0.978);ADVIA120测定的血小板计数、血小板压积和血小板分布宽度与CD3500均有显著差异(P<0.01),平均血小板体积无显著差异(P>0.05)。激光法测定血小板各参数正常参考范围分别为PLT男性(241±57.5)×109/L,女性(265±62.7)×109/L;PCT男性(0.185±0.04)%,女性(0.2±0.04)%;PDW男性(60.9±8.05)%,女性(58.7±7.5)%;MPV为(7.85±1.37)fl;MPC为275.5±15.25;MPM为2.04±0.275;LPLT为(7±3.5)×109/L。结论ADVIA120血细胞分析仪为检测血小板最理想的仪器之一。Objective To evaluate two-dimensional(2-D)platelet analysis and to calculate the normal range of Ml platelets parameters on the ADVIA120 hematology analyzer.Methods One hundred and seventeen samples from patients were processed for platelet count analysis using manual method,ADVIA120 and CD3500.One thousand and two samples from normal volunteers were processed by ADVIA120 for the normal range of platelet.Results They have no difference between manual method and ADVIA120.The normal range of platelet and its parameters are:PLT:male (241±57.5)×109/L,female (265±62.7)×109/L:PCT:male (0.185±0.04)%,female (0.2±0.04)%;PDW:male (60.9±8.05)%,female (58.7±7.5)%:MPV:(7.85±1.37)fl,MPC:275.5±15.25;MPM: 2.04 ±0.275;LPLT:7±3.5×109/L.Conclusion ADVIA120 is one of the best analyzer to measure platelet and its parameters.

关 键 词：血小板计数 激光法 手工法 

分 类 号：R4[医药卫生—临床医学]