应用单细胞凝胶电泳技术评价复合微生态制剂的遗传毒性  

Assessment of the Genotoxic Effects of Microorganism Compounds on Fish by Single Cell Gel Electrophoresis Assay

作　　者：周国勤[1] 杜宣[1] 吴伟[2] 

机构地区：[1]南京市水产科学研究所,江苏南京210029 [2]中国水产科学研究院淡水渔业研究中心,中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,江苏无锡214081

出　　处：《农业环境科学学报》2005年第z1期236-239,共4页Journal of Agro-Environment Science

基　　金：江苏省水产三项工程项目(PJ2003-1)

摘　　要：采用DNA损伤检测技术———单细胞凝胶电泳技术(SCGE,又名彗星试验)来检测复合微生态制剂对鱼类DNA的损伤,尝试利用鱼类的单细胞凝胶电泳技术来评价外来化学物质对水生生态环境的影响。试验结果表明,复合微生态制剂对鱼类的DNA没有明显的损伤作用,各试验浓度组与对照组相比没有显著性差异(P>0.05),复合微生态制剂对水体生态环境具有安全性。试验同时表明,鱼类肾细胞的DNA损伤可作为评价外来化学物质遗传毒性的生物标志物。The damage caused by the microorganism compounds to DNA of Carp^(')s kidney cells was estimated by using alkaline single cell gel electrophoresis assay(SCGE,namely comet assay).The method of SCGE of fish kidney cells was established to assess the risk of chemicals to the eco-environment.The results showed that the microorganism compounds didn't damage the DNA of the tested fishes.There were no significant differences of DNA damage between the tested groups and the negative control groups(P>0.05).So the microorganism compounds were safe to the aquatic ecosystems.The SCGE was a very sensitive method in determining the damage of DNA,and the damage of DNA could be considered as a biomarker in determining the environmental mutagens.

关 键 词：单细胞凝胶电泳技术 复合微生态制剂 鱼类 遗传毒性 

分 类 号：X826[环境科学与工程—环境工程]