毛细管电泳测定血清及结肠中地塞米松磷酸钠含量的方法学研究  

Study of the determination of the content of dexamethasone sodium phosphate in serum and colon by HPCE

作　　者：郭丹[1] 陈娜娜[2] 李国锋[1] 

机构地区：[1]第一军医大学南方医院药学部 [2]第一军医大学药物研究所,广东广州510515

出　　处：《中国现代应用药学》2005年第z1期629-631,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：国家卫生部归国世川生启动基金(批准号:055);广东省医学科研基金(A2002371)资助

摘　　要：目的建立测定血清及结肠中地塞米松磷酸钠(DSP)浓度的方法。方法高效毛细管电泳法。毛细管60cm×75μm;运行缓冲液100mmol·L^(-1)四硼酸钠(pH 9.2),高压进样5s,分离电压20kv,温度为25~0C,检测波长为240nm。三氯乙酸沉淀蛋白后上清液直接进样,测定血清及结肠中的浓度。结果血清及结肠中的DSP能得到较好分离,无明显干扰峰,DSP的线性范围为0.78~50μg·mL^(-1)(r=0.9995)。血清和结肠中平均提取回收率分别为89.51%和92.34%。结论该法简便、分离度好,灵敏度高,可用于DSP的药代动力学研究。OBJECTIVE To establish a method for determination of the content of dexamethasone sodium phosphate(DSP) in serum and colon.METHODS The HPCE was adopted.The best condition was achived with a fuse dsilca capillary tube(60cm ×75μm),100 mmol · L^(-1) sodium tetraborate buffer(pH 9.2),at a constant voltage of 20 kv and temperature at 25℃.The UV detection wavelength was at 240 nm.The proteins were precipitated with trichloroacetic acid,the sample was directly injected to determine the content of DSP in serum and colon.RESULTS DSP was well separated from any other obvious interactive peaks in serum or colon sample.The method showed a good linearity for DSP in the range of 0.78 ~ 50 μg · mL^(-1)(r = 0.9995),while the lowest limit of detection was 0.04 μg · mL^(-1).The average recoveries were obtained as 89.51%from serum sample and 92.34%from colon sample respectively.Conclusions The established HPCE method for determination of DSP will be very useful in the study of pharmacokineties of DSP.

关 键 词：地塞米松磷酸钠 血清 结肠 高效毛细管电泳法 

分 类 号：R917.794.5[医药卫生—药物分析学]