高效液相色谱法测定胡芦巴药材中葫芦巴碱的含量  

Determination of Trigonelline in Semen Trigonellae by HPLC

作　　者：郭增喜[1] 陈碧莲[1] 李文霞[2] 戚雁飞[1] 

机构地区：[1]浙江省药品检验所,浙江杭州310004 [2]国光药业有限公司,浙江杭州310018

出　　处：《中国现代应用药学》2005年第z1期641-642,共2页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立胡芦巴药材中葫芦巴碱含量测定的方法.方法采用离子对色谱法,以十八烷基硅烷键合硅胶为填充剂;甲醇-冰醋酸-0.05%十二烷基磺酸钠溶液(20:0.1:80)为流动相;检测波长为265nm;柱温30℃.结果葫芦巴碱进样量在0.07968～1.1952μg范围内有良好的线性关系,r=1.0000,平均回收率为102.44%,RSD为0.7%(n=6).结论此含量测定方法简便、可靠、重现性好,可作为胡芦巴药材含量测定的方法.OBJECTIVE To establish a HPLC method for detennination of trigonelline in Semen Trigonellae.METHODS A shimpack reversed-phase C_(18) analytical column(150×4.6mm I.D,5μm) was utilized at a column temperature of 30℃.The mobile phase was methanol-0.05%SDS- glacial acetic acid.The detection wavelength was 265 nm.RESULTS The calibration curve showed good linearity over the range of 0.07968-1.1952μg(r = 1.0000).The average recovery was 102.44%and the relative standard deviation was 0.7%(n=6).CONCLUSION The method is simple,and accurate with a good reproducibility and can be used as quantitative analysis method for Semen Trigonellae.

关 键 词：离子对色谱法 胡芦巴 葫芦巴碱 

分 类 号：R917.78.1[医药卫生—药物分析学]