乙型肝炎病毒C区变异与拉米夫定治疗后HBVDNA反弹关系的研究  

THE STUDY OF THE RELATIONSHIP BETWEEN THE C GENE MUTATIONS OF HBV AND HBVDNA REBOUND AFTER LAMIVUDINE THERAPY

作　　者：苏明华[1] 江建宁[1] 周元平[2] 陈茂伟[1] 刘志红[1] 玉艳红[1] 张鹭[1] 

机构地区：[1]广西医科大学第一附属医院感染性疾病科,南宁530021 [2]广州南方医院

出　　处：《广西医学院学报》2008年第3期358-360,共3页

基　　金：广西自然科学基金资助项目(No.0342009);广西卫生厅课题资助(No.Z2003043);海南省自然科学基金(No.80579)

摘　　要：目的:探讨乙型肝炎病毒(HBV)C区变异及变异位点与拉米夫定抗病毒治疗后HBVDNA反弹的关系。方法:应用PCR-序列分析法,多引物对巢式PCR法,检测10例用拉米夫定治疗的乙型肝炎患者(治疗组),以及10例从未用过抗病毒治疗患者(对照组)的C区的突变位点及HBV基因型。结果:治疗组10例病人中4例检出有C区变异,变异位点分别为:I134V、P164Q、I126L+E142D+P159T及L84I+I88V+I126L+P159A+P164;对照组10例病人中有5例检出有C区变异,变异位点分别为:A83G、A83G+P164QI、126L+P164Q、I126L+P159T;其中I134V、E142D、L84II、88V位点的变异仅出现在拉米夫定治疗后HBVDNA反弹的3例病人中,而且感染的HBV均为C基因型。结论:C区I134V、E142D、L84II、88V位点变异可能与拉米夫定的耐药有关;感染C基因型的病人比B基因型的病人更易出现与拉米夫定耐药相关的C区变异。Objective: To investigate the relationship between the C gene mutations of hepatitis B virus(HBV) and HBVDNA rebound after lamivudine therapy.Methods:10 patients of CHB with HBV DNA rebound after lamivudine therapy(therapy group) and 10 patients of CHB without any antiviral therapy(control group) were investigated.The Core gene mutation sites were identified by PCR gene sequence analysis and HBV genotype was detected by nested PCR with multiple pair primers in sera of 10 patients after HBV DNA rebound and i...

关 键 词：乙型肝炎病毒 基因型 C区变异 拉米夫定 HBVDNA反弹 

分 类 号：R512.62[医药卫生—内科学]