心肌细胞分离方法的改进及其钾电流的观察  

IMPROVEMENT OF MYOCARDIAL CELL ISOLATION AND OBSERVATION OF POTASSIUM CURRENT

作　　者：高分飞[1] 黄展勤[1] 周燕琼[1] 石刚刚[1] 

机构地区：[1]广东省汕头大学医学院药理学教研室,汕头515041

出　　处：《广西医学院学报》2008年第3期338-340,共3页

基　　金：国家自然科学基金(No.30672465);广东省自然科学基金重点项目(No.06118928);广东省医学科研基金(A2008435)

摘　　要：目的:探索建立简单可靠的心肌细胞的酶解分离方法,提高细胞存活率和膜片钳实验成功率。方法:采用Langendorff装置,无钙液和胶原酶P二步灌流法分离心肌细胞。显微镜下观察细胞形态结构,并应用膜片钳全细胞技术记录大鼠心室肌细胞膜瞬时外向钾电流。结果:心肌细胞存活率高,形态结构良好,可成功记录出瞬时外向钾电流。结论:通过改进的分离方法获得的心肌细胞存活率高,具有正常的电生理特性。Objective: To explore and establish a simple and reliable method of isolating single cardiac myocytes in order to improve the quantity and quality of cells for patch clamp research.Methods: The ventricular myocytes were isolated enzymatically by two-step perfusion with calcium-free solution and collagenase P on the Langendorff apparatus.The morphous and construction were observed under the microscope,and the transient outward potassium current was recorded by the use of patch clamp whole-cell recording tech...

关 键 词：心肌 细胞分离 膜片钳 瞬时外向钾电流 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]