不同培养液对卵母细胞体外成熟及重组卵母细胞孤雌活化的影响  

Effects of different culture media on in vitro materation of germinal vesicle oocytes and artificial activation of reconstructed oocytes

作　　者：周雪莹[1] 崔龙波[2] 黄秀英[3] 孙方臻[3] 

机构地区：[1]大连理工大学生物科学与工程系,大连116024 [2]烟台大学生物化学系,烟台264005 [3]中国科学院遗传与发育生物学研究所,北京100080

出　　处：《南京大学学报（自然科学版）》2008年第4期415-420,共6页Journal of Nanjing University（Natural Science）

基　　金：国家“973”项目(G1999055902)

摘　　要：为研究生发泡(GV)移植后的重组卵母细胞能充分在体外成熟和发育,比较了几种培养液对GV期卵母细胞体外成熟和重组卵母细胞孤雌活化的影响.自然获得的GV期卵母细胞和经显微操作获得的重组卵母细胞在M2、HTF和M199培养液培养,其体外成熟率无差异(P>0.05);但成熟的重组卵母细胞经人工活化后,生长在HTF培养液的细胞发育到2-细胞期胚的比率(49.2%)明显高于生长在M199培养液的比率(28.9%)(P<0.05).结果表明HTF培养液更适合小鼠卵母细胞的体外成熟和进一步发育.To study oocytes reconstructed by germinal vesicle(GV) translation can mature and develope fully in vitro,effects of different culture media on in vitro maturation of GV oocytes and artificial activation of reconstructed oocytes were compared.After pregnant mare s serum gonadotropin(PMSG) treatment,6-～8-week-old Kunming mice were killed to collect GV oocytes.GV oocytes obtained directly were cultured in M2,HTF and M199 media,the rates of germinal vesicle breakdown(GVBD)were observed,and the ratio of metapha...

关 键 词：培养液 卵母细胞 体外成熟 孤雌活化 

分 类 号：Q813[生物学—生物工程]