葡萄糖氧化酶在金胶修饰碳糊电极上的直接电化学及其应用  

Direct Electrochemistry of Glucose Oxidase on Colloidal Gold Modified Carbon Paste Electrode and Its Application

作　　者：郭晓明[1] 王根礼[1] 叶伟林[1] 周祖新[1] 朱贤[1] 

机构地区：[1]上海应用技术学院化学工程系,上海200235

出　　处：《上海应用技术学院学报（自然科学版）》2008年第2期85-89,共5页Journal of Shanghai Institute of Technology: Natural Science

基　　金：上海市教育委员会科技基金资助项目(06OZ001)

摘　　要：在制备金胶修饰碳糊电极(Au/CPE)的基础上,将葡萄糖氧化酶(GOD)通过吸附作用固定在Au/CPE表面。固定化的GOD与电极之间能够进行直接的电子传递,纳米金颗粒能增大对GOD的吸附量和吸附强度,同时使GOD的氧化还原中心FAD/FADH2的还原态FADH2更趋稳定。对修饰电极检测葡萄糖的机理进行了讨论,并制备了基于检测氧化电流的直接电子传递型葡萄糖传感器。Colloidal gold modified carbon paste electrode has been prepared.Glucose oxidase(GOD) is immobilized on the Au/CPE by absorption exhibiting a well-defined behavior of direct electron transfer.The magnitude of absorbed GOD is increased and the absorbility of GOD improved as the gold nanoparticles are introduced into CPE.Meanwhile,FADH2 adsorbed on Au/CPE is more stable than that absorbed on CPE.The detection mechanism of the modified electrode for glucose has been discussed and glucose sensor of direct elect...

关 键 词：金胶 碳糊电极 直接电子传递 葡萄糖氧化酶 葡萄糖传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]