三七总皂苷对大鼠海马神经细胞缺氧缺糖再给氧损伤的保护作用  

Protective Effects of Panax Notoginseng Saponins on Rat Hippocampal Neuron Injury Induced by Hypoxia/Hypoglycemia and Reoxygenation

作　　者：陈志刚[1] 朱陵群[2] 王席玲[2] 张壮[2] 闫彦芳[2] 牛福玲[2] 

机构地区：[1]北京中医药大学东方医院神经内科,北京100078 [2]北京中医药大学东直门医院中医内科学教育部重点实验室和脑病研究室,北京100700

出　　处：《中国实验方剂学杂志》2008年第5期55-58,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家人事部留学回国人员基金资助(1999037A)

摘　　要：目的:观察三七总皂苷对大鼠海马神经细胞缺氧缺糖再给氧损伤的保护作用。方法:建立缺氧/缺糖再给氧模型,模拟缺血再灌注损伤。流式细胞术检测凋亡细胞百分率,荧光显微镜观察细胞形态学变化和坏死细胞百分率,同时,测定细胞乳酸脱氢酶(LDH)的漏出和一氧化氮(NO)的产生量。结果:神经细胞缺氧/缺糖5 h后再给氧可诱导神经细胞凋亡和细胞坏死,并显著增加LDH的漏出和NO的产生,并随再给氧时间的延长而增加。三七总皂苷能降低神经细胞凋亡及坏死的百分率,减少LDH的漏出和NO的过量产生,三七总皂苷的作用随剂量增加而增加。结论:三七总皂苷具有拮抗海马神经细胞凋亡的作用,这种作用可能与降低或抑制一氧化氮合酶(NOS)活性,减少NO的过量产生有关。Objective:To observe the protective effects of Panax Notoginseng Saponins(PNS) on hypoxia /hypoglycemia and reoxygenation injury of rat hippocampal neurons.Methods: The damaging models of hypoxia /hypoglycemia and reoxygenation were established on cultured rat hippocampal neurons.Apoptosis were measured by flow cytometry.Morphological changes and neuronal necrosis were observed with fluorescence microscope.Nitric oxide(NO) contents and the leakage of lactic dehydrogenase(LDH) were measured.Results: hypoxia/...

关 键 词：三七总皂苷 神经细胞 凋亡 一氧化氮 缺氧/缺糖 

分 类 号：R285.5[医药卫生—中药学]