高效液相色谱法测定山楂叶提取物中熊果酸的含量  

Determination of Ursolic Acid in Hawthorn Leaves by HPLC

作　　者：卢文胜[1] 危华玲[2] 

机构地区：[1]广西医科大学附属第三医院,广西南宁530031 [2]广西壮族自治区人民医院,广西南宁530021

出　　处：《中国实验方剂学杂志》2008年第6期15-16,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西壮族自治区卫生厅课题(Z2003081)

摘　　要：目的:建立山楂叶提取物中熊果酸的含量测定方法。方法:高效液相色谱法,C18柱,以甲醇-水-磷酸(90∶10∶0.03)为流动相,流速为0.5mL·min-1,检测波长为220nm。结果:熊果酸在(0.982~9.82)μg·mL-1范围内与峰面积线性关系良好,相关系数为0.9998,平均回收率为98.16%,RSD为0.58%(n=6)。结论:方法简便,准确,重复性好,适用于山楂叶提取物的质量控制。Objective:To develop an HPLC method for determination of ursolic acid in Hawthorn Leaves. Methods:The C_ 18 column with methanol-water-phosphorous acid (90∶10∶0.03) as the mobile phase was used. The flow rate was 0.5 mL·min~ -1 and the detection wavelength was 220 nm. Results:The linear range of calibration curve was (0.982～9.82) μg·mL~ -1 for ursolic acid (r= 0.999 8 ). The average recovery was 98.16% and the RSD was 0.58% (n=6). Conclusion:The method is simple, accurate and repeatable. It can be used for ...

关 键 词：山楂叶 熊果酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]