反相高效液相色谱法测定炎立消胶囊中原儿茶酸、原儿茶醛和酪醇的含量  

Determination of Protocatechuic Acid,Protocatechuic Aldehyde and Tyrosol in Yanlixiao Capsules by RP-HPLC

作　　者：张淑蓉[1] 裴妙荣[1] 裴香萍[1] 黄灿林[1] 黄轩[1] 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《中国实验方剂学杂志》2008年第6期12-14,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:测定炎立消胶囊中原儿茶酸、原儿茶醛、酪醇的含量。方法:采用反相高效液相色谱(RP-HPLC)法直接测定。YWG-C18色谱柱(4.6mm×250mm,10μm);流动相甲醇-0.125%冰醋酸水溶液(10∶90),流速1.0mL·min-1;检测波长275nm。结果:原儿茶酸在0.08μg^0.56μg范围呈良好线性,回归方程为Y=85117x-15733,r=0.9995;原儿茶醛在0.01μg^0.07μg范围呈良好线性,回归方程为Y=111700x-37980,r=0.9998;酪醇在0.42μg^2.94μg范围呈良好线性,回归方程为Y=139386x-29146,r=0.9996。原儿茶酸回收率96.0%,RSD为2.37%(n=6);原儿茶醛回收率98.4%,RSD为2.05%(n=6);酪醇回收率97.5%,RSD为1.93%(n=6)。结论:该方法简便、快速、准确可靠,并可同时测定炎立消胶囊中原儿茶酸、原儿茶醛、酪醇多种成分的含量,适用于炎立消胶囊的质量控制。Objective:To develop a RP-HPLC method to determine the contents of protocatechuic acid, protocatechuic aldehyde and tyrosol in Yanlixiao Capsules. Methods: The samples was separated on a YWG C_ 18 column(4.6 mm×250 mm,10 μm), using methanol-0.125% glacial acetic acid solution(10∶90)as the mobile phase with flow rate of 1 mL·min~ -1 and detection at 275 nm. Results:The regression equation of protocatechuic acid was Y= 85 117x -15 733 (r= 0.999 5 ),and the linear range was at 0.08 μg～0.56 μg. The regression e...

关 键 词：炎立消胶囊 反相高效液相色谱 原儿茶酸 原儿茶醛 酪醇 

分 类 号：R286.0[医药卫生—中药学]