气相色谱法测定蜜炼川贝枇杷膏中薄荷脑和苯甲醛的含量  

GC Determination of Menthol and Benzaldehyde in Milian Chuanbei Pipagao

作　　者：杨敏[1] 刘吉金[1] 

机构地区：[1]深圳市药品检验所,广东深圳518029

出　　处：《中国实验方剂学杂志》2008年第8期23-25,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立蜜炼川贝枇杷膏中有效成分薄荷脑和苯甲醛的气相色谱测定法。方法:采用DB-1毛细管柱(0.32mm×30m×0.25μm),程序升温,柱温80℃,保持1min后,以10℃.min-1升至180℃后,继续以25℃.min-1升至250℃,保持5min,进样口温度为220℃,检测器温度为260℃,FID检测器,无分流进样。结果:薄荷脑在浓度(3.18～31.8)μg.mL-1范围内线性关系良好(r=0.9998),平均回收率为98.59%;苯甲醛在浓度(5.25～52.52)μg.mL-1范围内线性关系良好(r=0.9999),平均回收率为98.27%。结论:该法简便、准确、可用于蜜炼川贝枇杷膏中薄荷脑和苯甲醛测定方法。Objective:To establish a method for the determination of menthol and benzaldehyde in Milian Chuanbei Pipagao by GC with capillary column.Methods:A DB-1 quartz capillary column(0.32 mm×30 m×0.25 μm) was used.The temperature program was as following:the intial temperature of column was kept at 80℃ for 1 min,then raised to 180℃ at the rate of 10℃·min-1,finally to 250℃ at the rate of 25℃· min-1 and maintained the temperature for 5 min.The injection and detection temperature was 220℃ and 260℃.Splitless sampling ...

关 键 词：蜜炼川贝枇杷膏 薄荷脑 苯甲醛 气相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]