HPLC法测定清胰片中芦荟大黄素、大黄酸、大黄素、大黄酚的含量  

Determination of Aloeemodin,Rhein,Emodin and Chrysophanol in Qingyi Tablets by HPLC

作　　者：王洪志[1] 范俊婷[1] 刘勇[1] 樊华[1] 李婉晴[1] 

机构地区：[1]天津市南开医院,天津300100

出　　处：《中国实验方剂学杂志》2008年第9期13-15,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立高效液相色谱法(HPLC)同时测定清胰片中芦荟大黄素、大黄酸、大黄素、大黄酚的含量。方法:色谱柱为TIANHE-C18(4.6 mm×250 mm,10μm),流动相:甲醇-0.1%高氯酸(75∶25),检测波长254 nm,流速为1.0 mL.min-1,柱温为25℃。结果:芦荟大黄素、大黄素在(0.0624~0.312)μg.mL-1范围内线性良好;芦荟大黄素r=0.9999、大黄素r=0.9992;大黄酸、大黄酚在(0.156~0.780)μg.mL-1范围内线性良好,大黄酸r=0.9998、大黄酚r=0.9999;样品平均回收率为:芦荟大黄素99.31%,RSD=1.71%;大黄酸99.65%,RSD=0.56%;大黄素99.10%,RSD=1.82%;大黄酚99.51%,RSD=1.24%。结论:该方法可靠、准确,专属性强,重复性好,可用于该制剂质量控制。Objective:To determine the contents of aloeemodin,rhein,emodin and chrysophanol in Qingyi Tablets by HPLC.Method: C_(18) column was used with mobile phase consisted of methanol-0.1%.Perchloric acid solution(75∶25),at a flow rate of 1.0 mL·min^(-1).The detecting wavelength was set at 254nm.Result: The linearity of this method was good in the ranges of((0.062 4)～(0.312))μg for aloeemodin(r=(0.999 9));(0.156～0.780) μg for rhein(r=(0.999 8));((0.062 4)～0.312)μg for emodin(r=(0.999 2));(0.156～0.780) μg for chrys...

关 键 词：清胰片 高效液相色谱法 芦荟大黄素 大黄酸 大黄素 大黄酚 

分 类 号：R286[医药卫生—中药学]