稀针嗜蓝孢孔菌多糖分析方法研究  

Study on Determination of Polysaccharide in Fomitiporia robusta(P.Karst.) Fiasson

作　　者：朱胤龙[1] 陈萍[1] 张亚环[1] 

机构地区：[1]陕西省中医药研究院,陕西西安710003

出　　处：《中国实验方剂学杂志》2008年第9期1-3,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局新药开发专项课题(D2X024A);陕西省科学技术研究发展计划项目(2002K11-G6(4))

摘　　要：目的:用紫外分光光度法建立稀针嗜蓝孢孔菌多糖的定量分析方法。方法:采用苯酚-硫酸法,以葡萄糖含量为指标,用L9(34)正交试验法选择最佳显色条件,检测波长为488 nm。结果:苯酚、浓硫酸二因素对多糖含量有显著影响,以葡萄糖表示,本法在(3.223~12.89)μg.mL-1范围内,吸收度与浓度有良好的线性关系,平均回收率96.60%,RSD为1.71%。结论:本法快速、简便,灵敏度、重复性好,不受蛋白质干扰,亦可用于混有糖苷的粗多糖的测定。Objective:To establish UV method to determine polysaccharide in Fomitiporia robusta(P.Karst.) Fiasson.Method:The parameter of colorimetric method was optimiged through L_9(3~4) orthogonal design with the index of glucose,by the use of phenyl hydrate-sulfuric acid,at the 488 nm.Result:The significant effects of phenyl hydrate-sulfuric acid volume on glucose content were discovered.The average of recovery was 96.6% and RSD 1.71% for glucose with in good linear relation in(3.223～12.89)μg·mL^(-1) with a correla...

关 键 词：稀针嗜蓝孢孔菌 多糖 正交试验 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]