山羊FSH及其长效类似物基因在小鼠乳腺中的暂态表达  

Transient expression of goat FSH and its long-acting analogous gene in mouse mammary gland

作　　者：严正杰[1] 杨欢利[1] 孙雪萍[1] 赵西彪[1] 丁家桐[1] 

机构地区：[1]扬州大学动物科学与技术学院

出　　处：《中国兽医学报》2008年第7期862-866,共5页Chinese Journal of Veterinary Science

基　　金：江苏省自然科学基金资助项目(BK2005050)

摘　　要：应用山羊促卵泡素长效类似物基因构建其乳腺暂态表达载体pcDNAFSH-βCTP、pcDNAFSH-βCTP-α及构建报告基因pcDNAEGFP,测序后通过脂质体转染至怀孕末期的小鼠乳腺组织。结果表明,构建的乳腺暂态表达载体在小鼠乳腺中获得了表达,表达量分别为pcDNAFSHα,β(7.933±2.074)IU/L,pcDNAFSHα,-βCTP(9.311±2.995)IU/L,pcDNAFSH-βCTP-α(11.059±4.107)IU/L。Transient expression vector pcDNAFSHβ-CTP,pcDNAFSHβ-CTP-α were constructed by using goat follicular stimulation hormone and the report gene vector pcDANEGFP was constructed.Then the vectors were transfected into the mouse mammary gland tissue at the end of pregnancy by using liposome after gene sequencing.The results showed that the transient expression vector were expressed in the mouse mammary gland for pcDNAFSHα,β,pcDNAFSHα,β-CTP,pcDNAFSHβ-CTP-α with their expressing amount of(7.933±2.074) IU/L,(9.311±2....

关 键 词：山羊 促卵泡素长效类似物基因 报告基因 小鼠乳腺 暂态表达 

分 类 号：Q78[生物学—分子生物学]