内毒素诱发睢宁白山羊实验性乳腺炎模型的建立及黄芪多糖对其的影响  

Establishment of experimental mastitis model by endotoxin in Suining white goat and effect of astragalus polysaccharide on it

作　　者：钟凯[1] 王艳玲[1] 刘仪[2] 邹思湘[2] 陈伟华[2] 

机构地区：[1]河南农业大学生理生化实验室,河南郑州450002 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《中国兽医学报》2008年第7期844-847,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30371049);河南省杰出人才创新基金资助项目(0121000300)

摘　　要：选择同处于泌乳初期的睢宁白山羊6头,体质量30~40 kg,C.M.T.法检测每头山羊2个乳区乳的体细胞数(SCC)均低于5×105/mL。每头山羊每天早上挤奶后,于右乳区经乳头管灌注黄芪多糖(APS)5 mL,左乳区则通过乳头管灌注等量生理盐水(PSS)作为对照,连续灌注5 d。第6天早上挤奶后,按50μg/kg剂量于左、右乳区通过乳头管注入大肠杆菌内毒素,分别于内毒素处理前1 h及处理后1、2、3、4、5、6、7、8、9、24 h观察乳房的变化并记录直肠温度;并于内毒素处理前1 h、处理后3、6、9、24 h取颈静脉血和采集左、右2个乳区的乳样,采用放射免疫法测定乳汁及血清中TNF-α和IL-2的水平;内毒素处理后24 h,处死动物,取乳腺组织,Bouin氏液固定,H.E.染色进行病理组织学观察。临床症状与病理切片观察均显示,通过乳导管将50μg/kg的内毒素灌注到山羊的乳腺内迅速诱导出典型的急性乳腺炎症状。灌注APS的乳区,乳中TNF-α的水平内毒素处理后3 h显著高于灌注PSS的乳区,92、4 h显著低于灌注PSS的乳区(P<0.05)。与内毒素处理前相比,灌注APS的乳区在内毒素处理后,乳中IL-2...Six Suining white goats in their early lactation,weighing 30-40 kg were used in this study.Before experiment,total quarter milk SCC were measured with California Mastitis Test(C.M.T.).The individual quarter SCC were less than 500 000 cells/mL.The mammary right quarters of 6 goats were infused with about 5 mL of APS.Left quarters were used as controls and infused with 5 mL of sterile saline.The administration of APS had been continuing for five consecutive days.On the sixth day,after morning milking,the mamm...

关 键 词：黄芪多糖 山羊 乳腺炎 内毒素 细胞因子 

分 类 号：S858.23[农业科学—临床兽医学]